摘要
目的：观察姜黄素对佐剂性关节炎大鼠成纤维样滑膜细胞（FLS）细胞增殖、细胞周期和周期蛋白D1的影响.方法：体外原代培养佐剂性关节炎大鼠FLS.取第3代FLS分别加入浓度为10,20,40,80 μmol/L的姜黄素溶液,共同培养24 h、72 h.MTT法检测药物对FLS增殖的影响,流式细胞术分别分析细胞周期和周期蛋白D1.结果：共培养24 h后40、80 μmol/L浓度组的MTT光密度值与空白对照组比较,差异有统计学意义（P〈0.05）,80 μmol/L浓度组G1期细胞所占比例和周期蛋白D1荧光指数与空白对照组差异也有统计学意义（P〈0.05）;共培养72 h 后10,20,40,80 μmol/L各浓度姜黄素组的光密度值、G1期细胞所占比例及周期蛋白D1与空白对照组比较,差异有统计学意义（P〈0.05）.结论：姜黄素能抑制佐剂性关节炎大鼠纤维样滑膜细胞的增殖,使细胞停滞在G1期,该作用可能与降低周期蛋白D1的表达有关.
Objective： To observe the effect of curcumin on rats with adjuvant arthritis fibroblast-like synovial cells （FLS） cell proliferation, cell cycle and the impact of cyclin D1. Methods： Primary culture of rat adjuvant arthritis FLS. Passage 3 were added to the concentration of FLS 10,20,40,80 μmol/L of curcumin solution, incubated 24 h, 72 h. M33 assay of drugs on FLS proliferation, cell cycle analysis by flow cytometry, respectively, and cyclin D1. Results： After 24 h, 40,80 μmol/L concentrations of MTT optical density compared with the control group, the difference was statistically significant （P〈0.05）, 80 μmol/L concentrations and the proportion of G1 phase cells Cyclin D1 fluorescence index of the control group also had a significant difference （P〈0.05）; were cultured 72 h, 10,20,40,80 μmol/L of various concentrations of curcumin group of optical density, G1 phase cell percentage and the proportion of cyclin D1 compared with the control group, the difference was statistically significant （P〈0.05）. Conclusion： Curcumin can inhibit adjuvant arthritis in rats fiber-like synovial cell proliferation, cell arrest at G1 phase, may reduce the role of cyclin D1 expression.
出处
《中医药导报》
2010年第8期92-94,共3页
Guiding Journal of Traditional Chinese Medicine and Pharmacy
