摘要
昆虫的普通气味结合蛋白(general odorant binding protein,GOBP)是气味结合蛋白(odorant binding protein,OBP)家族中的重要成员,与昆虫感受低特异性气味分子刺激相关,在觅食、求偶等生理行为过程中发挥重要的作用。基于家蚕5龄第3天幼虫的基因芯片和蛹期、成虫期的RT-PCR表达谱分析发现,家蚕的GOBP1蛋白基因(gobp1)主要在幼虫的头部及蛹和成虫的触角中表达,在幼虫的睾丸以及成虫的胸足、体壁、脂肪体等非感受器官中也有表达,而GOBP2蛋白基因(gobp2)的表达谱和gobp1相比明显较窄。该结果表明家蚕gobp1以感受气味分子刺激的功能为主,同时还可能具有其他尚未被发现的生理功能;而家蚕gobp2可能具有对气味感受更高、更专一的功能特点。对家蚕及其他12种鳞翅目昆虫的GOBP蛋白序列比对发现,各物种间的GOBP蛋白序列相似性很高,蛋白二级结构元件也高度相似,具有的昆虫OBP典型结构中的半胱氨酸(C)位点非常保守。基于非同义突变与同义突变比值(Ka/Ks)的分子进化分析显示,13种鳞翅目昆虫的GOBP蛋白基因仅有棉铃虫gobp1在分化中受到正向选择作用,揭示不同昆虫的GOBP蛋白基因可能具有相似的生理功能。
General odorant binding proteins （GOBPs） are important members of the odorant binding protein （OBP） family in insects.They are involved in stimulus reception of low specificity odorous molecules,and possess considerable significances in the progress of foraging and courtship.Based on expression profiles of microarray analysis to 5th instar day 3 silkworm （Bombyx mori） larvae and reverse transcription-polymerase chain reaction （RT-PCR） to silkworm pupae and adults,we found that silkworm gobp1 gene was expressed predominantly in larval heads,pupal antennae,and ephebic antennae,and so did in non-perceptive organs such as larval testis,ephebic integument,and fat body with lower expression levels.Compared with gobp1,the expression profiles of gobp2 were narrower significantly.Those results suggested that GOBP1 mainly functions in stimulus perception of odorous molecules.Meanwhile,it has other unknown physiological functions.On the contrary,GOBP2 is characterized by much higher degree of chemoreception depend-ability compared with GOBP1.Sequence alignment of GOBP proteins from silkworm and other 12 lepidopteran insect species showed that GOBP sequences had high sequence similarity across species and the proteins＇ secondary structure elements were also highly similar.The cysteine sites in OBP typical structure of insects were ex-tremely conservative.Molecular evolution analysis based on the ratio of nonsynonymous （Ka） and synonymous （Ks） of 13 insect species showed that there was at most one positive selection site,indicating that GOBP genes may possess similar physiological functions in different insects.
出处
《蚕业科学》
CAS
CSCD
北大核心
2010年第4期610-618,共9页
ACTA SERICOLOGICA SINICA
基金
现代农业产业技术体系建设专项(蚕桑)
关键词
家蚕
普通气味结合蛋白
基因表达
分子进化
基因芯片数据
Bombyx mori
General odorant binding protein
Gene expression
Molecular evolution
Microarray
