摘要
以红麻细胞质雄性不育系(GMS)L23A、保持系L23B及从该保持系中发现的细胞核雄性不育系(CMS)L23BS为材料,采用石蜡显微制片技术,在光学显微镜下比较观察了三者花药发育过程中小孢子的发育过程及组织化学变化。结果表明,L23A的花药发育过程中,小孢子发育的不同阶段均出现败育现象,最早的败育表现为花粉母细胞在减数分裂之前的退化解体,最终形成空的花粉囊;有的因花粉母细胞减数分裂过程异常而导致不能形成小孢子四分体;有的因小孢子在四分体中不能正常释放而败育,同时绒毡层细胞过度液泡化,并提早解体死亡;花药发育早期含有少量蛋白质和淀粉,随着花药的发育逐渐变少。而L23BS小孢子败育的时期集中于四分体至单核花粉期间,表现为小孢子发育异常,有些小孢子不能从四分体里释放出来而影响其正常发育。在四分体以前与可育系类似,花药含丰富的蛋白质和淀粉。在败育的过程中,花药中的蛋白质和淀粉含量渐渐减少,但药隔组织中的颗粒淀粉含量几乎不变。
Kenaf（Hibiscus cannabinus L.） is a potential alternative crop developed for fiber production,but the utilization of hybrids of the sterile line combination in production was seldom reported.Nuclear male sterile lines were discovered from the maintainer line L23B by Dr.Zhou Ruiyang in 2005,and six cytoplasmic male sterile（CMS） lines including cytoplasmic male sterile（CMS） line L23A were identified in 2007.For exploring the mechanism of male sterility,it is necessary to study the changes of some metabolic materials in the developing anther.Microsporogenesis of cytoplasmic male sterile（CMS） line L23A,together with its maintainer line L23B and genic male sterile（GMS） line L23BS which is a mutant of L23B,was studied by microscopic observation with cytological and cytochemical methods.The results indicated that microspore deformation of cytoplasmic male sterile（CMS） lines L23A occurred at different stages.Some anthers contained degenerated pollen mother cells（PMCs） which might result in empty pollen sac;some PMCs failed to pass meiosis;some microspores could not be released from tetrad successfully;and tapetal cells vacuolated.Cytochemical studies indicated there were a little protein and a few starch grains in the young anthers.During the growth of anther,no protein and less starch were observed.In genic male sterile（GMS） line L23BS,some microspores were unable to escape from tetrad and aborted mainly between tetrad and mononuclear pollen stages.Before then protein and starch deposited as normal which disappeared gradually after tetrad stage.It should be noted that the protein and starch disappeared gradually,but the content of big starch grains in L23BS connective did not changed during its microsporogenesis.
出处
《作物学报》
CAS
CSCD
北大核心
2010年第8期1414-1424,共11页
Acta Agronomica Sinica
基金
国家自然科学基金项目(30471106)资助
关键词
红麻
细胞核雄性不育
小孢子发生
细胞形态学
组织化学
Kenaf（Hibiscus cannabinus L.）
Genic male sterile
Microsporogenesis
Cytomorphology
Cytochemistry
