摘要
利用亚硝化细菌分离培养基从湿地浅层底泥中分离到一株具有硝化反硝化能力的异养硝化细菌,经过常规的分析和部分长度的16S rDNA序列分析表明,分离所得HB-1菌株与多株Micrococcus sp.16S rDNA具有99%的相似性,初步鉴定为Micrococcus sp.。分别采用乙酸钠和氯化铵作为碳源和氮源进行硝化特性研究,结果表明,8d的好氧培养,铵态氮去除率为49.2%,并且具有产生亚硝态氮的硝化性能。以硝酸钠为氮源进行好氧培养,总氮没有去除效果;而6d的缺氧静置培养,总氮的去除率达90.7%,表现明显的反硝化作用。
By using nitrosobacterial selective medium, a heterotrophic nitrobacterium strain HB-1 with nitrification-denitrification capability was isolated from wetland surface sediment. The conventional staining analysis and the phylogenetic analysis based on 16S rDNA showed that the strain HB-1 had 99% similarity to Micrococcus sp. 16S rDNA, and was preliminarily identified as Micrococcus sp. Taking sodium acetate as the carbon source and ammonium chloride as the nitrogen source, an 8-day aerobic incubation test showed that the NH4-N removal rate by the strain was 49.2%, and trace of NO2-N was detected. Taking sodium nitrate as the sole N source, the strain could not remove N under aerobic incubation, but could remove 90.7% of N after 6-day anoxic incubation, reflecting its important role in denitrification.
出处
《生态学杂志》
CAS
CSCD
北大核心
2010年第8期1629-1633,共5页
Chinese Journal of Ecology
基金
国家重点基础研究发展计划项目(2006CB403306)
河南省科技攻关资助项目(082102220014)
关键词
异养硝化细菌
好氧硝化
缺氧反硝化
系统发育分析
heterotrophic nitrification bacteria
aerobic nitrification
anoxic denitrification
phylogenetic analysis
