摘要
目的以羟基萘作为生物标志物,了解幼儿园儿童萘的内暴露水平。方法于2007年11月,采集我国南方某市幼儿园4～6岁儿童尿样。尿液经过酶解、固相萃取(SPE)富集、净化,采用高效液相色谱-荧光检测器检测2-羟基萘浓度。结合问卷调查,分析尿中羟基萘浓度的影响因素。结果所有尿样均检出2-羟基萘和1-羟基芘。2-羟基萘含量为2.33～50.4μmol/molCr,中位数为7.30μmol/molCr。尿中1-羟基芘与2-羟基萘浓度呈正相关(r=0.328,P<0.01);而个体差异、是否被动吸烟和饮食均不会对儿童尿中2-羟基萘浓度产生显著影响。结论本次调查的儿童尿2-羟基萘浓度较高,可能与居住和生活环境的萘暴露有关。
Objective To explore the exposure level of naphthalene among children in kindergarten. Methods The urine samples were collected from children aged 4-6 years in a kindergarten in a city in South China in November, 2007. After enzymic hydrolysis, the urine samples were pre-eoncentrated and cleaned up by solid phase extraction, and then the urinary hydroxyl- naphthalenes and 1-hydroxypyrene were determined by high performance liquid chromatography with fluorescence detection. The levels of 2-naphthol in urine samples were developed to analyze the influence of some factors. Results 2-naphthol and 1-hydroxypyrene were detected in all urine samples. The concentration of 2-naphthol ranged from 2.33 to 50.4 μmol/mol creatinine, and median of concentration was 7.30 μmol/mol creatinine. There was a positive correlation between the levels of 2-naphthol and 1-hydroxypyrene in urine samples （r=0.328, P〈0.01）. The differences of concentration of 2-naphthol in urine were not significant among individuals, and passive smoking, food and drink were not the significant influencing factors. Conclusion The concentrations of urinary 2-naphthol among the investigated children in kindergarten are higher, and may be involved in exposure to naphthol in living and inhabiting environment.
出处
《环境与健康杂志》
CAS
CSCD
北大核心
2010年第7期608-610,共3页
Journal of Environment and Health
基金
中国科学院知识创新工程重要方向项目(KZCX2-YW-403)
关键词
多环芳烃
羟基萘
生物标志
高效液相色谱
尿液
Polycyclic aromatic hydrocarbons
Naphthol
Biomarker
High performance liquid chromatography
Urine
