摘要
目的:采用高脂饲料喂养法建立大鼠高脂血症模型,研究卷柏提取部位(A,B,C,D)对此大鼠模型肝脏的影响。方法:健康雄性Wistar大鼠70只,除正常对照组10只大鼠给予基础饲料外,其余均给予高脂饲料喂养,4周后尾部采血,测量血液中总胆固醇(TC)、三酰甘油(TG)的含量。根据TC值将大鼠随机分为模型组、阳性对照组、卷柏A组、卷柏B组、卷柏C组和卷柏D组(n=10)。连续给药16周后处死,给药的同时给予高脂饲料。计算大鼠的体重增长情况和肝脏指数,检测血清中丙氨酸氨基转移酶(ALT)、天冬氨酸转氨酶(AST)和肝脏中TC,TG,高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、脂联素(APN)、肿瘤坏死因子-α(TNF-α)、极低密度脂蛋白(VLDL)的水平。结果:给药16周后,卷柏A组和D组的ALT,AST,TC,TG,TNF-α水平均显著低于模型组(P<0.05,P<0.01),APN,VLDL水平均显著高于模型组(P<0.05,P<0.01),并且D组用药量明显低于A组,相当于A组用药量的1/4。结论:卷柏提取物对高脂血症大鼠肝脏有一定的调节作用,能降低脂肪在肝脏中积聚,有保护肝脏的作用。卷柏提取物减少脂肪在肝脏中积聚、改善肝脏脂肪变的作用可能与其提高肝脏中VLDL水平有关;卷柏提取部位D可能是其主要活性部位。
Objective： To evaluate the effect of Selaginella tamariscina（Beauv.） Spring extract（A,B,C,D） on liver functions in rats with hyperlipemia induced by high-fat diet.Methods： Seventy male Wistar rats were fed with normal or high-fat diets for 4 weeks.Four weeks later,serum TC and TG were assayed.According to the TC,hyperlipemic rats were randomly divided into 6 groups： hyperlipidemia control,positive control and 4 Selaginella extract（A,B,C,D） groups（n=10 in each group）.Rats were sacrificed after treatment for 16 weeks.Weight gain,liver index,serum ALT and AST levels,and liver TC,TG,HDL-C,LDL-C,APN,TNF-α and VLDL levels were assayed.Results： After 16-week administration of Selaginella extracts,ALT,AST,TC,TG,TNF-α levels were reduced in Selaginella extract groups A and D（P〈0.05 and 〈0.01）;and APN and VLDL levels were increased in Selaginella extract groups A and D（P〈0.05,P〈0.01） in comparison to the hyperlipidemia control group.The effective dose was significantly lower in group D than in group A,which was only 1/4 of the dose of group A.Conclusion： Selaginella extracts protects the liver in hyperlipidemic rats through regulating liver function and reducing the accumulation of fat in the liver.The mechanism may relate to elevation of liver VLDL level by the Selaginella extracts.The group D may be the active part.
出处
《中国新药杂志》
CAS
CSCD
北大核心
2010年第14期1216-1221,共6页
Chinese Journal of New Drugs
基金
国家重大新药创制科技重大专项项目(2009ZX09103-440)
河南省重大公益性科研招标项目(081100910800)
关键词
卷柏提取物
高脂血症
肝脏脂肪变
肝脏保护
Selaginella tamariscina（Beauv.） Spring extract
hyperlipemia
hepatic steatosis
hepatoprotection
