摘要
为提高中草药饲料添加剂的抑菌效果和应用效果,从中草药青蒿、鱼腥草、地骨皮、黄岑、胆草、夏枯草等中草药中筛选到1株能发酵中草药饲料添加剂的菌株,鉴定为米曲霉Y-1。以抗菌活性和CMCase酶、FPA酶及蛋白酶酶活为评价指标,研究了以青蒿、鱼腥草、地骨皮、胆草等为主组成的中草药饲料添加剂的发酵条件。结果表明,合适的发酵条件为:中草药饲料添加剂与麦麸的比例为7∶3、葡萄糖1%、发酵温度32℃、初始pH自然、物料含水量50%～60%、发酵周期为72h。在此条件下,发酵料蒸煮液对大肠杆菌、金黄色葡萄球菌、普通变形杆菌和鼠伤寒沙门氏菌的抑菌活性分别较对照提高36.03%、60.83%、27.64%、41.32%,CMCase、FPA酶和蛋白酶酶活分别达到9653、971U/g和6357U/g。说明中草药饲料添加剂通过微生物发酵可提高其作用效果。
To improve the effects of antibacterial and application of Chinese herbal feed additive.A Chinese herbal feed additive fermenting strain was isolated from Chinese herbal such as Herba artemisiae annuae ,Herba houttuyniae, Cortex Lycii,Scutellaria baicalensis Georgi, Corallodiscus flabellate and Spica prunellae etc.It was preliminarily identified as Aspergillus orzyme Y-1.The fermentation conditions for Chinese herbal feed additive containing Herba artemisiae an- nuae ,Herba houttuyniae ,Cortex Lycii,Corallodiscus flabellate were studied with the antibacterial activities and CMCase activity,FPA activity,proteinase activity as the indexes of evaluation.The results showed that the optimal fermentation conditions were：the ratio of Chinese herbal feed additive to wheat-bran was 7：3 ,glucose was 1% ,fermentation tempera- ture was 32 ~C ,initial pH was nature,water content was 50 % - 60 % ,fermentation time was 72 h.Under these condi- tions,the antibacterial activities of the fermented products were enhanced 36.03 % ,60.83 % ,27.64 % ,41.32 % against Escherichia coli,Saphylococcus aureus ,Proteus vulgaris ,Salmonella tyhimurium than the comparison. CMCase activity, FPA activity and the proteinase activity were respectively achieved 9653,971 U/g and 6357 U/g.The results indicated that the effects of Chinese herbal feed additive could improve by microbial fermentation.
出处
《中国饲料》
北大核心
2010年第15期13-17,共5页
China Feed
基金
湖南省教育厅资助(06C405)
关键词
中草药
饲料
饲料添加剂
发酵条件
Chinese herb
feed
feed additive
fermentation condition
