摘要
目的观察原癌基因C-myc、H-ras及抑癌基因p16在硫酸铍致人胚肺成纤维细胞(HELF)损伤下的表达,探讨硫酸铍对离体培养HELF的影响。方法不同物质的量浓度硫酸铍(终物质的量浓度为2.0、20.0、100.0μmol/L)重复染毒HELF后,采用免疫组化SP法和甲基化特异性聚合酶链式反应(MSP)技术分别检测C-myc、H-ras蛋白及p16基因的表达。结果不同物质的量浓度染毒组培养至第12、24、36天时,C-myc蛋白的表达均明显高于对照组,差异有统计学意义(P<0.05,P<0.01),且具有剂量效应关系(r12=0.755,r24=0.822,r36=0.792,P<0.01);不同物质的量浓度染毒组在培养至第24、36天时H-ras蛋白表达明显高于对照组,差异有统计学意义(P<0.05或P<0.01),且具有剂量效应关系(r24=0.586,r36=0.845,P<0.01)。染毒组p16基因在培养至第24、36天时,第24天2.0μmol/L染毒组表达部分甲基化条带,其他均表达甲基化条带。结论硫酸铍在引起HELF损伤的情况下,可诱发HELFC-myc和H-ras蛋白异常表达、p16基因甲基化。
Objective To observe the expressions of the related oncogene protein and anti-oncogene methylation with human embryonic lung fibroblasts （HELF） induced by beryllium sulfate.Methods After HELF were repeatedly administered with BeSO4 at different concentrations（final concentration 2.0,20.0,100.0 μmol/L）,immunohistochemistry technique was used to observe the expressions of oncogenes （C-myc,H-ras）.MSP method was introduced to test the methylation status of anti-oncogene （p16）.Results Compared with the control,the expressions of C-myc elevated in the dose groups at day 12,24 and 36,showing statistical differences （P0.05 or P0.01） and dose-response relationships （r12=0.755,r24=0.822,r36=0.792,P0.01）.H-ras protein also elevated at day 24 and 36,with significant statistically differences （P0.05 or P0.01） and dose-response relationships （r24=0.586,r36=0.845,P0.01）.Except the low concentration of BeSO4 group at day 24,gene p16 methylation bandings were produced at day 24 and 36.Conclusion BeSO4 could induce abnormal expressions of C-myc,H-ras and methylation of gene p16.BeSO4 could have carcinogenicity to humans.
出处
《中国职业医学》
CAS
北大核心
2010年第4期271-273,276,共4页
China Occupational Medicine
基金
宁夏教育厅资助项目(N29347)
宁夏自然科学基金项目(NZ997)
