摘要
目的:建立咽速康气雾剂中胆酸的含量测定方法。方法:利用HPLC-ELSD法,采用Cromasil C18色谱柱(4.6mm×250mm,5μm);流动相甲醇-0.1%冰醋酸水溶液梯度洗脱;漂移管温度75℃;氮气流速33psi。结果:胆酸对照品在0.6542～16.355μg内,进样量与峰面积间的对数值呈良好的线性关系,r=0.9998,平均回收率100.27%,RSD为2.13%。结论:本方法结果准确,重复性好,可作为该制剂的含量测定方法。
Objective：To establish the content determination method for cholic acid in Yansukang by HPLC-ELSD. Method：An HPLC method was applied with Kromasil-C18 column （4. 6 mm × 250mm,5 μm） by a gradient elution using A （ methanol）-B （0. 1% glacial acetic acid solution） as the mobile phase. The drift tube temperature was set at 75 ℃ ,and N2 pressure was 33 psi. Result：The linear range of cholic acid was 0. 6542-16. 355 μg;The correlation coefficient （r） was 0. 999 8,and the average recovery was 100. 27% ,with RSD 2. 13% （n = 6）. Conclusion：The method is accurate and reproducible,and can be used to determine the content of cholic acid in Yansukang.
出处
《中国实验方剂学杂志》
CAS
北大核心
2010年第10期91-92,95,共3页
Chinese Journal of Experimental Traditional Medical Formulae
