摘要
目的观察过食酸味药山茱萸对大鼠胃窦黏膜D细胞和内皮素(ET-1)、一氧化氮(NO)的影响。方法 60只大鼠随机分为空白对照组、山茱萸临床等效剂量组、山茱萸低、中、高剂量组,分别给予蒸馏水和山茱萸水煎液灌胃。连续给药9周后,进行胃组织病理形态学检查,免疫组织化学法检测胃窦D细胞数,放射免疫法测血浆ET-1含量,硝酸还原法测血清NO含量。结果低、中、高剂量组逐渐出现大鼠活动减少、倦卧嗜睡、食量减少、便溏、体质量减轻等中医脾虚症状。与空白对照组比较:(1)低、中、高剂量组的血浆ET-1含量、D细胞数及强阳性细胞率均明显升高,胃黏膜白细胞浸润程度明显加重,差异均具有统计学意义(P<0.05,P<0.01);(2)中、高剂量组血清NO值明显增高,差异有统计学意义(P<0.05)。结论胃窦D细胞增多以及ET、NO含量升高,可能为过食酸味药导致脾虚的内在病理机制,为《内经》"味过于酸,肝气以津,脾气乃绝"提供了实验依据。
Objective To explore the effects of overtaking the sour-flavored Chinese drug cornus officinalis on D Cells of gastric mucosa,ET-1 and NO in rats.Methods 60 SD rats were divided randomly into control group,clinical equivalent dose group of cornus officinalis,and low,middle and high dose groups of cornus officinalis,and all of them were given water or decocted cornus officinalis liquid respectively 9 weeks.The histological changes were measured,the D cells were detected with immunicytochemistry,the contents of plasma ET -1 with radioimmunoassay and the level of serum NO with reduction method.Results In the low,middle and high dose groups,there gradually appeared the symptoms of spleen deficiency syndrome,such as activity reduction,tiredness,drowsiness,low appetite,loosing stool,weight reduction,et al.Compared with the control group：① The plasma ET-1 and the number of D cells in low,middle and high dose groups increased obviously（P0.05）,the intensity of chronic inflammation significantly increased（P0.05,P0.01）;② The contents of serum NO in middle and high dose groups increased obviously（P0.05）.Conclusion Over feeding the sour cornus officinalis may lead to spleen deficiency syndromes in rats,which may be related to increasing the contents of ET-1,NO in blood and the number of D cells of the gastric mucosa.It provides evidence for Huangdi Neijing saying ＂Overtaking sour-flavored would make liver-qi excessive and exhaust spleen-qi.＂
出处
《湖南中医药大学学报》
CAS
2010年第7期19-21,共3页
Journal of Hunan University of Chinese Medicine
基金
中医临床基础重点学科建设资助项目
关键词
山茱萸
脾虚
胃窦D细胞
内皮素
一氧化氮
大鼠
cornus officinalis
spleen dificiency syndrome
D cells
ET-1
NO
rats
