摘要
目的：采用紫外分光光度法测定维生素B1片中维生素B1 的含量并对该方法进行验证.方法：用紫外分光光度法在246nm处测定吸光度,并用百分吸收系数法和对照品比较法计算.结果：用紫外分光光度法测定维生素B1 含量的方法专属性好;当维生素B1 浓度在7.5～17.5μg/ml范围时线性关系良好,回归方程为Y=0.0411X＋0.0094,线性相关系数r=1;平均回收率为99.60%,RSD=0.45%;精密度为0.76%;用百分吸收系数法计算平均标示量为101.7%,用对照品比较法计算平均标示量为104.3%.结论：用紫外分光光度法测定维生素B1片中维生素B1的含量操作简便、快速、准确、精密度好.
Objective：Uses ultraviolet spectrophotometric method determination Vitamin B 1 piece of Vitamin B 1 content and carries on the confirmation to this method. Methods：With the ultraviolet spectrophotometric method in the 246nm place determination extinction, and absorbs the method of correlates and the comparison comparison test computation with the percentage. Results： Determines the Vitamin B 1 content with the ultraviolet spectrophotometric method the method specificity to be good;When Vitamin ]3 ; density when 7. 5-17. 5 μg/ml scopes the linear relations is good, the regression equation is Y= 0.041 1＋ 0. 0094, linear correlation coefficient r= 1 . The average returns-ratio is 99.60%, RSD=0. 45 % ; The accu- racy is 0.76% ;Absorbs the method of correlates computation average indication quantity with the percentage is 101.70%, with the comparison comparison test computation average indication quantity is 104.3%. Conclusion：Determines Vitamin B with the ultraviolet spectrophotometric method 1 piece of Vitamin B 1 content operation simple,fast,accurate,the accuracy good.
出处
《按摩与康复医学》
2010年第24期1-2,共2页
Chinese Manipulation and Rehabilitation Medicine
关键词
紫外分光光度法
维生素B1
含量测定
Ultraviolet spectrophotometric method Vitamin B 1 Content determination
