摘要
目的:比较不同品种附子生物碱和多糖的含量。方法:采用酸性染料比色法测定附子总生物碱含量,反相高效液相色谱法测定双酯型生物碱含量,蒽酮-硫酸法测定多糖含量。结果:不同品种附子总生物碱和双酯型生物碱含量差异较大,附子多糖含量差异不显著。附子炮制后,黑附片中总生物碱、双酯型生物碱含量显著下降,附子多糖含量稍有增加。结论:本试验结果可为附子品种选育提供一定的科学依据。
OBJECTIVE： To research the content of alkaloids and polysaccharides in different varieties of Aconitum carmichaeli. METHODS： The contents of total alkaloids, diester diterpenoid alkaloids and polysaccharides were measured by acid dye colorimetry, HPLC and sulphuric acid-anthrone colorimetric method respectively. RESULTS： In different varieties, the differences of total alkaloids and diester diterpenoid alkaloids were significant. However, the difference of polysaccharides wasn’t significant. The content of total alkaloids and diester diterpenoid alkaloids in processed A. carmichaeli reduced significantly while the content of polysaccharides increased slightly. CONCLUSION： The results of study could provide a scientific basis for rational breeding of A. carmichaeli.
出处
《中国药房》
CAS
CSCD
北大核心
2010年第31期2916-2918,共3页
China Pharmacy
基金
国家支撑计划课题(2007BAD89B15)
四川省农作物育种攻关课题(2006YZGG12-22)
关键词
附子
品种
总生物碱
双酯型生物碱
多糖
含量
Aconitum carmichaeli
Varieties
Total alkaloids
Diester diterpenoid alkaloids
Polysaccharide
Content
