摘要
目的探讨5-羟色胺4(5-HT4)受体调节剂(激动剂和抑制剂)对肝部分切除(PH)后大鼠胃肠道5-羟色胺(5-HT)和肝再生的影响。方法 60只成年SD大鼠PH后分为对照组、西沙必利(激动剂)组和GR113808(抑制剂)组3组;西沙必利组PH后每12h按10mg/kg体重的西沙必利灌胃,GR113808组PH后每12h按3mg/kg体重的GR113808腹腔注射;分别于PH后0h、24h、48h、72h计算肝/体重比,并取血液、胃、小肠和肝组织;用免疫组织化学技术显示胃肠中5-HT免疫阳性(5-HTIR)细胞,用图像分析系统测定胃肠5-HTIR细胞平均灰度,用酶联免疫吸附法(ELESA)检测血液中的5-HT,用银染技术显示肝细胞核仁组织区相关嗜银蛋白(AgNORs)。结果与对照组比,1.西沙必利组胃肠中5-HTIR细胞数于PH后48～72h显著下降(P<0.05)、细胞的灰度于PH后24～72h显著上升(P<0.05),血中5-HT的含量于PH后24～72h显著上升(P<0.05),肝/体重比和肝组织AgNORs颗粒数在PH后48～72h显著增加(P<0.05);2.GR113808组在PH后24～72h期间,5-HTIR细胞数较对照组无显著性差异,但细胞的灰度显著下降(P<0.05或P<0.01),血中5-HT的含量显著下降(P<0.05或P<0.01),肝/体重比于48～72h显著下降(P<0.05或P<0.01),肝中AgNORs颗粒数于24～72h显著减少(P<0.05或P<0.01)。结论用5-HT4受体调节剂改变胃肠道5-HT的分泌量,可导致肝/体重比和肝细胞的转录活性发生相应的改变;胃肠道分泌的5-HT具有促进肝细胞增殖的作用。
Objective To investigate the relationship of 5-HT in gastrointestinal tract with liver regeneration using 5-HT4 receptor regulator in rats after partial hepatectomy （PH）. Methods Adult SD rats （n = 60） with PH were divided into three groups： control, cisapride and GR113808 group. After PH, Cisapride （ a 5-HT4 receptor agonist, 10 mg/kg, twice per day, ig） and GR113808 （a 5-HT4 receptor antagonist, 3 mg/kg, twice per day, ip） were administered to the corresponding groups, respectively. Four kind of tissues （blood, stomach, small intestine and liver） were taken out at 0 hour, 24 hours, 48 hours, 72 hours, respectively, then the ratios of liver mass to body weight was accounted. 5-HT immunoreactive cells （5-HTIR cells） in stomach and small intestine were detected by immunohistochemical technique. The average gray level of 5-HTIR cells was detected by image analysis system. The plasma 5-HT level was determined by enzyme-linked immunosorbent assary（ELESA）. The numbers of argyrophil nucleolar organizer regions （AgNORs） were detected by silver staining technique. Results Compared with the control, 1. The amounts of 5-HTIR cells in both stomach and small intestine decreased at 48-72 hours （P 〈 0. 05）, the gray level of 5-HTIR cells increased significantly at 24-72 hours when plasma 5-HT level increased significantly（P 〈 0. 05 ） , both the ratios of liver mass to body weight and AgNORs particles numbers in liver increased significantly at 48-72 hours （ P 〈 0.05 ）, in cisapride rats with PH ; 2. Theamounts of 5-HTIR cells in both stomach and small intestine had not statistical difference at 24-72 hours when both the gray level of 5-HTIR ceils and plasma 5-HT level decreased significantly （ P 〈 0.05 or P 〈 0. 01 ） , the ratios of liver mass to body weight decreased significantly at 48-72 hours, and AgNORs particle numbers in liver decreased significantly at 24-72 hours （P 〈 0.05 or P 〈 0.01 ） , in GRl13808 rats with PH. Conclusion Secretion of 5-HT in gastr
出处
《解剖学报》
CAS
CSCD
北大核心
2010年第4期592-597,共6页
Acta Anatomica Sinica
基金
河南省基础与前沿技术研究计划项目资助(82300450550)
