摘要
目的探讨癌基因DJ-1在甲状腺癌中的表达效应及其意义。方法采用免疫组织化学方法 (SABC法)检测74例甲状腺癌和10例正常甲状腺组织中DJ-1蛋白的表达,分析DJ-1蛋白在正常甲状腺组织与甲状腺癌之间的表达差异,以及4种甲状腺癌病理类型间的表达差异。结果 DJ-1在正常甲状腺组织中未见表达,而在甲状腺癌中呈显著表达(94.6%);33例乳头状癌中DJ-1全部呈阳性表达(100.0%),19例滤泡癌中有17例呈阳性表达(89.5%),13例髓样癌中有12例呈阳性表达(92.3%),9例未分化癌中有8例呈阳性表达(88.9%)。DJ-1表达水平在甲状腺癌不同病理类型间比较未见统计学差异(P>0.05)。结论癌基因DJ-1特异性表达于甲状腺癌组织中,可能参与甲状腺癌的发生,但与其发展及预后无关。
Objective To investigate the expression of oncogene DJ-1 and its significance in thyroid cancer.Methods The expression of DJ-1 protein in 74 cases of thyroid cancer and 10 cases of normal thyroid tissue were detected by streptavidin-biotin complex method.Results No expression of DJ-1 protein was found in normal thyroid tissue,but it was strongly expressed in thyroid cancer tissue,with a positive expression rate of 94.6%.The positive expression rates of DJ-1 were 100.0% in 33 cases of papillary thyroid cancer,89.5% in 19 cases of follicular thyroid cancer,92.3% in 13 cases of medullary thyroid cancer,and 88.9% in 9 cases of undifferentiated thyroid cancer.No significant difference in the expression of DJ-1 was found among different pathological types of thyroid cancer.Conclusion DJ-1,an oncogene,is expressed specifically in thyroid cancer.It may be involved in the pathogenesis of thyroid cancer,but has no relationship with the development and prognosis of thyroid cancer.
出处
《中国医科大学学报》
CAS
CSCD
北大核心
2010年第7期549-551,共3页
Journal of China Medical University
基金
国家自然科学基金资助项目(30870522)
辽宁省科技攻关项目(2007225021)
辽宁省教育厅高校科研基金资助项目(2009A765)
