摘要
采用PCR-SSCP技术,对秦川牛(QC)、鲁西牛(LX)、南阳牛(NY)、郏县红牛(JXR)4个品种共计459个个体BMPR-IB基因第8外显子进行SNPs多态性检测,并分析基因型与部分体尺性状的相关性。在QC、LX和NY中分别检测到AA、AB和BB 3种SSCP多态型,且QC、LX和NY 3个品种中A和B等位基因的频率分别为0.4949/0.5051、0.4663/0.5337和0.4118/0.5882。测序结果显示,A等位基因与GenBank公布的序列(NC_007304.3)相比在43 933位发生T→C突变。最小二乘法分析表明,3个品种中BB基因型个体的体高显著高于AA和AB基因型个体(P<0.05);在LX和NY中,BB型个体的腰高显著高于AA和AB型个体(P<0.05);体长方面,LX中BB型个体大于AA和AB型个体(P<0.05),NY中BB型个体大于AB型个体(P<0.05),但与AA型个体差异不显著(P>0.05);3种基因型对黄牛其他体尺性状没有显著影响(P>0.05)。结果表明,BMPR-IB基因对黄牛体尺性状有一定影响,可以作为黄牛标记辅助选择的候选基因。
PCR-SSCP technology was applied to analyze the polymorphisms of exon 8 of BMPR-IB gene in 459 yellow cattle,including Qinchuan cattle（QC）,Luxi cattle（LX）,Nanyang cattle（NY） and Jiaxian Red cattle（JXR） breeds.Three BMPR-IB genotypes（AA,AB,BB） were identified in QC,LX and NY.The allelic frequencies of A and B in three cattle breeds were 0.4949/0.5051,0.4663/0.5337 and 0.4118/0.5882,respectively.The sequencing results showed that A allele had a base mutation of T→C at the 43 933th bp contrasting with the NC_007304.3 of cattle.Statistical analysis indicated that in QC,LX and NY,body height of individuals with genotype BB was significantly higher than that of individuals with genotype AA and AB respectively（P0.05）.In LX and NY,waist height of individuals with genotype BB was significantly higher than that of individuals with genotype AB and AA respectively（P0.05）.In LX,body length of individuals with genotype BB was higher than those with genotype AA and AB respectively（P0.05）.In NY,body length of individuals with genotype BB was significantly higher than that of individuals with genotype AB（P0.05）,but there was no significant difference between BB and AA（P0.05）.Analyzed genotype did not show any significant effect for the other traits（P0.05）.It was concluded that BMPR-IB gene has some effect on body measurement traits and can be used as candidate gene for marker-assisted selection in cattle.
出处
《西北农业学报》
CAS
CSCD
北大核心
2010年第7期12-16,共5页
Acta Agriculturae Boreali-occidentalis Sinica
基金
国家863计划项目(2006AA10Z1A1)
国家科技支撑计划(2006BAD01A10-3)
国家转基因重大专项(2008ZX08007-002)
陕西省13115科技创新工程(2007ZDCY-01)
关键词
黄牛
BMPR-IB
体尺性状
SNP
Yellow cattle
BMPR-IB gene
Body measurement traits
Single nucleotide polymorphism（SNP）
