摘要
目的探讨HE4 mRNA表达与卵巢癌临床病理特征关系和临床意义。方法利用RT-PCR方法检测对照组(n=21)、卵巢交界性肿瘤(n=21)和卵巢癌(n=51)组织中HE4 mRNA表达,应用放射免疫法定量测定卵巢癌患者血清CA125含量。结果在卵巢交界性肿瘤和癌组中,HE4 mRNA和血清CA125阳性表达明显高于对照组(P<0.01,P<0.05),HE4 mRNA阳性表达的特异性明显高于血清CA125(P<0.05)。在早期卵巢癌中,HE4 mRNA阳性表达的敏感性明显高于血清CA125(P<0.05)。结论在卵巢癌中HE4 mRNA表达上调,与卵巢癌的发生和病理生物学行为关系密切,可成为区分卵巢良、交界性和恶性肿瘤的重要标志。
Objective To investigate the relationship between the mRNA expression of HE4 and clincopathological features in ovarian cancer and it＇s clinical Significance.Methods We examined the mRNA expression of HE4 and serum levels of CA125 in control group（n=21）,ovarian borderline tumor（n=21） and epithelial ovarian cancer（n=51） by RT-PCR and electrochemiluminescence method.Results The mRNA expression of HE4 and Serum levels of CA125 were significantly higher in ovarian borderline tumor and ovarian cancer than that in control group（P0.01,P0.05）.The specificity and sensitivity of the mRNA expression of HE4 in ovarian borderline tumor and the sensitivity in earlier ovarian cancer and the specificity in ovarian cancer were significantly higher than serum level of CA125 respectively（P0.05）.Conclusion Increased mRNA expression of HE4 were closely associated with tumorigenesis and pathobiological behaviors of ovarian cancer,therefore may be used as specific tumor markers for the detection of the benign and borderline tumor and l ovarian cancer.
出处
《中国误诊学杂志》
CAS
2010年第23期5548-5551,共4页
Chinese Journal of Misdiagnostics
基金
辽宁省自然科学基金项目(编号:20092102)
辽宁省教育厅基金(编号:20060961)
