摘要
为了解地处多环芳烃(PAHs)排放源附近的背景人群体内的PAHs暴露水平,于2006年11月采集广东某市某钢铁公司焦化厂附近一中学部分学生(14～18岁)尿样,采用高效液相色谱—荧光检测法,在检测尿液中PAHs单羟基代谢物(HO-PAHs)的基础上,对受试人群体内的PAHs暴露水平,1-羟基芘(1-HOP)与其他HO-PAHs的相关性进行了分析。结果表明,尿样中共检测出7种HO-PAHs,除4-羟基菲(4-HOPhe)外,其余HO-PAHs的检出率均为100%。检出的2-羟基萘(2-HON)、2-羟基芴(2-HOFlu)、2-+3-羟基菲(2-+3-HOPhe,由于2-羟基菲(2-HOPhe)和3-羟基菲(3-HOPhe)属共溢出峰,因此以2-+3-HOPhe表示)、9-羟基菲(9-HOPhe)、4-HOPhe和1-HOP的浓度中位数分别为5.34、4.09、0.08、0.80、0.03、0.35μmol/mol(以尿肌酐计)。受试人群尿液中2-HON、2-HOFlu、总HOPhe浓度(简称∑HOPhe)和1-HOP的浓度中位数高于南方沿海某市中学生,1-HOP浓度的中位数也高于国内类似背景人群。1-HOP浓度与其他HO-PAHs浓度的相关性并不好(相关系数为0.213～0.653),且与2-HON、9-HOPhe和检出的∑HOPhe浓度的相关性均不显著,显示多种HO-PAHs的联合测定可能更能客观评价人体内的PAHs暴露水平。
In order to detect the hydroxy-PAHs （HO-PAHs） level in people exposured to PAHs pollution, some urine samples of student （14-18 years） from a school nearby the coke and chemical plant were collected in November 2006. The urinary HO-PAHs were determined by high performance liquid chromatography with fluorescence detection, base on the testing results, the human internal exposure level of PAHs and the correlation between 1-hydroxypyrene （1-HOP） and other HO-PAHs were analyzed. Seven HO-PAHs were detected in urinary samples, the detection frequencies of HO-PAHs were 100% except for 4-hydroxy phenanthrene （4-HOPhe）. The median concentrations of those metabolites were （μmol/mol creatinine） ： 2-hydroxy naphthalene （5. 34）, 2-hydroxy fluorene （4.09）, 2-＋ 3-hydroxyl phenanthrene（0.08）, 9 hydroxy phenanthrene（0.80）, 4-hydroxy phenanthrene（0.03） and 1 hydroxy pyrene （0.35）. The median concentration of 2-HON, 2-HOFlu, total HOPhe（ ∑ HOPhe） and 1-HOP in test urine samples was higher than those of other students in South China, and for 1-HOP, the median concentration was higher than the reported data from similar background population in China. There were relatively poor correlations between 1-HOP and other HO-PAHs, especially for 2-hydroxy naphthalene （2 HON）, 9 HOPhe and HOPhe, indicating that multi-determination of HO-PAHs might be more objective to assess the PAHs level on human exposure.
出处
《环境污染与防治》
CAS
CSCD
北大核心
2010年第8期47-50,共4页
Environmental Pollution & Control
关键词
多环芳烃
单羟基代谢物
高效液相色谱
尿液
polycyclic aromatic hydrocarbons （PAHs）
hydroxy polyeyclic aromatic hydrocarbons（HO-PAHs）
high performance liquid chromatography
urine
