摘要
为研究板蓝根、甘草和鱼腥草3种中药粗提物体外抗猪繁殖与呼吸综合征病毒(porcine reproductive and respiratory syndrome virus,PRRSV)的作用,采用体外细胞培养法,在Marc-145单层细胞上,对3种中药粗提物进行直接抗PRRSV活性试验.结果表明,板蓝根和甘草粗提物阻断PRRSV的最佳质量浓度分别为3.125、0.391mg/mL,抑制PRRSV感染的最佳质量浓度分别为3.125、0.782mg/mL,直接灭活PRRSV的最佳质量浓度分别为1.563、0.782mg/mL;鱼腥草粗提物质量浓度为0.782mg/mL时,阻断和抑制PRRSV感染的效果最好,但对PRRSV没有直接灭活作用.3种中药粗提物均有不同程度的体外抗PRRSV活性作用,其中板蓝根的效果最好.
Antiviral activity of isatis tinctoria, glycyrrhiza and houttuynia cordata extraction against porcine reproductive and respiratory syndrome virus was estimated in the monolayer Marc-145 cell. The result showed that as for precaution , inhibition and the direct killing function, the best concentration of isatis tinctori were 3.125, 3.125 and 1.563 mg/mL; the optimum concentration of glycyrrhiza were 0.391, 0.782 and 0.782 mg/mL, respectively. The optimum concentration of houttuynia cordata for precaution and inhibition was 0.782 mg/mL, while with on the direct killing function. It indicated that all of these three Chinese traditional medicines had different degrees of antiviral activity against PRRSV and the isatis tinctoria behaved the best in these chinese traditional medicines.in vitro.
出处
《湖南农业大学学报（自然科学版）》
CAS
CSCD
北大核心
2010年第4期459-463,共5页
Journal of Hunan Agricultural University(Natural Sciences)
基金
广东省科技计划项目(2008A020100020)
广东省自然科学基金(34064)
东莞市高等院校科研机构科技项目(2007108101114)
关键词
板蓝根
甘草
鱼腥草
猪繁殖与呼吸综合征病毒
isatis tinctoria
glycyrrhiz
houttuynia cordata
porcine reproductive and respiratory syndrome virus
