摘要
以蝴蝶兰花梗为试验材料,研究不同浓度的6-BA和NAA组合对腋芽诱导的影响,并应用正交试验设计,研究了基本培养基、6-BA、NAA和椰乳浓度对萌发腋芽增殖的影响,并进行了生根培养。结果表明,蝴蝶兰花梗腋芽诱导的适宜培养基为MS+6-BA5.0mg/L+NAA0.2mg/L+椰乳150mL/L,诱导率可达90.7%;萌发腋芽增殖的适宜培养基为MS+6-BA8.0mg/L+NAA0.2mg/L+椰乳150mL/L,增殖倍数达6.8;适宜的生根培养基为1/2MS+NAA0.4mg/L+活性炭1mg/L,生根率达93.3%。
With Phalaenopsis flower stalk as explant, the effect of different combines of 6-BA and NAA on the axillary bud induction was studied.The orthogonal designed experiment was used to study the effect of basic medium component, the concentration of 6-BA, NAA and coconut milk on the buds propagation, and rooting culture was carried.The result showed that the suitable medium for the axillary bud induction was MS＋6-BA5.0 mg/L ＋NAA0.2 mg/L＋coconut milk 150 mL/L,the induction rate reached 90.7%; the suitable medium for the adventitious buds propagation was MS＋6-BA8.0 mg/L＋NAA 0.2 mg/L＋ coconut milk 150 mL/L,the coefficient of reproduction was up to 6.8; the suitable medium for rooting was 1/2MS＋NAA 0.4 mg/L＋active carbon 1 mg/L,the rooting rate was 93.3%.
出处
《湖北农业科学》
北大核心
2010年第7期1547-1549,共3页
Hubei Agricultural Sciences
基金
菏泽学院科学研究基金项目(XY07SW01)
关键词
蝴蝶兰
离体再生条件
优化
Phalaenopsis Bl.spp.
in vitro cultivation conditions
optimization
