摘要
采用ＳＯＦ和ＴＣＭ１９９与输卵管上皮细胞共同培养系统对牛体外受精卵进行培养，观察了在这两种培养条件下得到的囊胚经冷冻—解冻后的孵化能力．在ＳＯＦ＋输卵管上皮细胞的培养条件下，囊胚的发育率为２１．３％，囊胚经冷冻—解冻后的孵化率为３７．５％；在ＴＣＭ１９９＋输卵管上皮细胞的培养条件下，囊胚的发育率为２６．０％，囊胚经冷冻—解冻后的孵化率为４７．７％．研究结果表明这两种体外培养系统的培养效果无显著差异（Ｐ＞０．０５）．
Bovine IVF embryos were cultured to blastocyst stage in SOF+BOEC and TCM199+BOEC respectively,the hatching of frozen thawed blastocysts were observed.In SOF+BOEC group,the rate of blastocysts was 21.3%,and the hatching rate of frozen thawed blastocysts was 37.5%.In TCM199+SOF group,the rate of blastocysts 26.0%,and the hatching rate of frozen thawed blastocysts was 47.7%.The results showed that the culture effects in those two culture systems are not significant difference ( P >0.05).
出处
《内蒙古大学学报（自然科学版）》
CAS
CSCD
1999年第2期219-222,共4页
Journal of Inner Mongolia University：Natural Science Edition
基金
国家863计划
关键词
牛
体外受精
胚胎
冷冻保存
孵化能力
解冻
bovine IVF embryos
in vitro development
embryo freezing
hatching ability
