摘要
目的:建立以柱前衍生化高效液相色谱-荧光检测法测定高血压患者尿液中22种氨基酸浓度的方法。方法:衍生化试剂为洗丹脱磺,流酰速氯为,色1.0谱m柱L·为mKinr-o1m,发asi射lC波18,长流为动5相08为nm甲,醇激与发3波0长mm为o3l·3L0-n1m醋,酸柱钠温缓为冲室溶温液,(进p样H量7.2为)-5甲0μ醇L-。四结氢果呋:2喃2(种84氨:1基5酸:1)检,测进浓行度梯度分别在5～200μmol·L-1范围内线性关系良好(r>0.9900);平均回收率为86.1%～109.0%;进样精密度RSD为0.84%～3.1%(n=6)。结论:本方法可同时测定22种氨基酸的浓度。
OBJECTIVE：To establish an HPLC method for the determination of 22 kinds of free amino acids in urine of hypertensive patients.METHODS：The determination was performed on a Kromasil C18 column with mobile phases of methanol and 30 mmol·L^-1 sodium acetate buffer（pH 7.2）-methanol-tetrahydrofuran（84：15：1）（gradient elution）.Flow rate was set at 1.0 mL·min^-1.The fluorescence excitation and emission wavelengths were 330 and 508 nm,respectively.Column temperature was room temperature and injection volume was 50 μL.Dansyl chloride was used as derivatization agent.RESULTS：The linear range of free amino acids were 5～200 μmol·L^-1（r〉0.990 0） with an average recovery ranged from 86.1% to 109.0%.The injection RSD were within 0.84%～3.1%（n=6）.CONCLUSIONS：The method is successfully applied for simultaneous determination of 22 kinds of free amino acids.
出处
《中国药房》
CAS
CSCD
北大核心
2010年第30期2823-2825,共3页
China Pharmacy
