摘要
目的评价迈瑞BC-5500血细胞分析仪定量分析外周血白细胞域未成熟细胞(LIC)和异常淋巴细胞(ALY)的性能。方法选200例BC-5500LIC报警值大于3%、200例BC-5500ALY报警值大于3%的阳性样本和100份BC-5500ALY及LIC均无报警值的阴性样本。分别采用BC-5500和镜检分析LIC%和ALY%,对BC-5500和镜检结果进行比较。各选取5份不同ALY%和LIC%水平的样本进行BC-5500批内重复性评价,分别选取8份标本在4℃和室温进行样本稳定性评价。结果 200例LIC报警的阳性标本BC-5500分析LIC%结果与镜检结果的符合率是26.5%,200例ALY报警的阳性标本BC-5500分析ALY%结果与镜检结果的符合率是69.5%;LIC%和ALY%的假阳性率分别为73.5%、30.5%。100例LIC和ALY均无报警的阴性标本,BC-5500分析LIC%和ALY%结果与镜检结果的符合率分别是96%和68%。5份不同LIC%和ALY%水平样本重复性的变异系数(CV%)分别从3.22%到6.25%和3.25%到6.12%;BC-5500分析4℃和室温保存24h内的样本结果稳定。结论 BC-5500血细胞分析仪的重复性及稳定性良好;但定量分析外周血LIC和ALY的准确性差,显示出较高的假阳性及假阴性,该仪器幼稚细胞报警功能尚需不断完善。
Objective To evaluate the performance of measurement of large immature cell（LIC） and atypical lymphocyte（ALY） in peripheral blood using BC-5500 hematology analyzer.Methods Each 200 positive samples with LIC and ALY alarm value3% were selected to compare BC-5500 count and manual morphology count;100 negative samples without alarm were selected to compare BC-5500 and the manual morphology count.5 samples at different levels were selected to evaluate within-run reproducibility for BC-5500 LIC% and ALY% count,8 specimens were selected to evaluate the sample stability at 4℃ and at room temperature.Results In 200 positive samples,the comparison analysis showed LIC% coincidence rate was 26.5% between BC-5500 count and manual morphology count.In 200 positive samples the comparison analysis showed ALY% coincidence rate was between BC-5500 count and manual morphology count,the falsepositive rates was 73.5% and 30.5%.In 100 negative samples without alarm,comparison analysis showed LIC% and ALY% coincidence rate was 96% and 68% between BC-5500 count and manual morphology.The within-run reproducibility performed using fresh blood on 5 different LIC% level specimens showed a range from 3.22% to 6.25% CV and the ALY% specimens showed a range from 3.25% to 6.12% CV.The percent of LIC% and ALY% were stable at both room temperature and after refrigeration for 24 h.Conclusion In the performance of quantitative measurement of LIC and ALY in peripheral blood using BC-5500 hematology,accuracy is poor,showing a high false positive and false-negative,the instrument alarm function for immature cells still needs to be improved for meeting the requirements of clinical laboratories.
出处
《检验医学与临床》
CAS
2010年第15期1580-1581,1583,共3页
Laboratory Medicine and Clinic
关键词
未成熟细胞
异常淋巴细胞
血细胞分析仪
定量分析
large immature cell
atypical lymphocyte
hematology analyzer
quantitative analysis
