摘要
目的:观察巨噬细胞移动抑制因子(MIF)和血管内皮细胞生长因子(VEGF)在卵巢癌组织中的表达并计数微血管密度(MVD),探讨MIF和VEGF与卵巢癌发生的关系及在卵巢癌血管生成中的作用。方法:利用免疫组织化学染色技术检测MIF、VEGF和第八因子相关抗原在卵巢癌组织和正常卵巢组织中的表达;采用ImageProPlus(IPP)6.0图像分析软件对结果进行分析。结果:MIF和VEGF在正常卵巢组织的实质细胞中不表达,在卵巢癌肿瘤细胞的胞浆呈阳性表达,且随着卵巢癌分化程度的降低,表达强度逐渐增强;低分化卵巢癌与高分化和中分化卵巢癌比较差异有显著性(P<0.05或P<0.01)。低分化卵巢癌组织中的MVD(50.83±11.59)明显增加,与正常卵巢组织(18.16±2.26)、高分化(19.88±3.92)及中分化(31.1±3.08)卵巢癌组织比较差异有显著性(均P<0.01)。MIF表达与VEGF表达呈正相关(r=0.5797,P<0.01),MIF表达与卵巢癌MVD呈正相关(r=0.9623,P<0.001)。结论:MIF的表达与卵巢癌的分化程度密切相关,MIF可能通过上调VEGF表达促进卵巢癌的肿瘤血管生成。
Objective To investigate the expressions of macrophage migration inhibitory factor（MIF）and vascular endothelial growth factor（VEGF）in ovarian cancer tissues and detect microvascular density（MVD）and elucidate the correlations between MIF,VEGF and the occurrence of ovarian cancer and explore their roles in tumor angiogenesis.Methods The expressions of MIF,VEGF and factor Ⅷ related antigen in normal ovarian tissues and ovarian cancer tissues were detected by immunohistochemical staining.The results were analyzed by Image Pro Plus（IPP）6.0 software.Results There were no visible expressions of MIF and VEGF in normal ovarian tissues,but positive expressions in ovarian cancer.The expressions of MIF and VEGF were increased significantly with the decreasing grade of the ovarian cancer.There were significant differences of the IOD values between well-differentiated,moderately differentiated and poorly differentiated ovarian cancer（P〈0.05 or P〈0.01）.The MVD in poorly differentiated ovarian cancer tissues（50.83±11.59）was significantly higher than those in normal ovarian tissues（18.16±2.26）,well differentiated（19.88±3.92）,moderately differentiated（31.1±3.08）ovarian cancer tissues（all P〈0.01）.There was positive correlation between the expressions of MIF and VEGF in ovarian cancer tissues（r=0.579 7,P〈0.01）.Moreover,the expression of MIF was positively correlated with MVD in ovarian cancer tissues（r=0.962 3,P〈0.01）.Conclusion MIF plays an important role in the oncogenesis and development of ovarian cancer,and it may promote the angiogenesis of ovarian cancer tissues by up-regulating the expression of VEGF.
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2010年第4期713-716,I0002,共5页
Journal of Jilin University：Medicine Edition
基金
吉林省科技厅科技发展计划项目资助课题(200705369,200705373)
关键词
巨噬细胞移动抑制因子
血管内皮细胞生长因子
微血管密度
血管新生
卵巢肿瘤
macrophage migration inhibitory factor
vascular endothelial growth factor
microvascular density
angiogenesis
ovarian cancer
