摘要
目的：提供简便有效的骨髓细胞（ＢＭＣ）保存方法，以便进一步提高骨髓移植效果。方法：以昆明种小鼠急性放射病为模型，进行了－８０℃冷冻保存ＢＭＣ及其程序移植的研究。结果：－８０℃保存的ＢＭＣ其有核细胞回收率为（８９．０±３．５）％，锥虫蓝拒染率为（８５．０±３．６）％；产率（每１０５个ＢＭＣ）：单核巨噬细胞克隆形成单位（ＣＦＵ－ＧＭ）为１０８±１０，脾细胞克隆形成单位（ＣＦＵ－Ｓ）为１２±３。昆明种小鼠程序移植－８０℃保存的同种异基因ＢＭＣ４×１０５，其活存率与一次移植同条件冻存的ＢＭＣ１×１０７相同（３０％）；程序移植４×１０６时，活存率显著提高（６０％）。经性染色体检查各组均有植入证据。结论：－８０℃保存ＢＭＣ是一种行之有效的方法；骨髓程序移植可能充分利用了每次腾出的“龛位”，减少了移植细胞数，却增加了干细胞植入，提高了移植效果，是一种“种子与土壤”
Objective: To enhance the efficiency of bone marrow transplantation (BMT) and to provide a simple and effective method for cryopreservation of bone marrow cells (BMCs). [WTHZ]Methods: BMCs were cryopreservated at -80℃ and a new method for bone marrow scheduled transplantation (BMST) was studied with acute radiation disease model of Kunming mice. Results: The method for cryopreservation of BMCs at -80℃ produced the highest recovery rates for nucleated cells (89 0%±3.5%), the trypan blue exclusion (85 0%±3.6%), CFU GM [(108±10)/10 5 BMC], CFU S[(12±3)/10 5 BMC]. Using this method, lethally irradiated Kunming mice received allogenetic BMST. The survival rate of mice (30%) in BMST group transplanted with 4×10 5 BMCs was the same as that in once BMT group transplanted with 1×10 7 BMCs. And the survival rate of mice (60%) in BMST transplanted with 4×10 6 BMCs was significantly higher than that in once BMT. Evidence of engraftment could be detected in all groups. Conclusion: This method for cryopreservation BMCs at -80℃ is simple and effective, and these data suggest that “Niches” vacated each time could be fully used in BMST, so the transplanted BMCs could be decreased, the engraftment of stem cells could be increased, and the grafting efficiency could be enhanced. This method is a new method aiming at “both seed and soil”.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
1999年第2期69-70,共2页
Academic Journal of Second Military Medical University
基金
国家自然科学基金
关键词
骨髓移植
冷冻保存
辐射损伤
bone marrow transplantation
niche
freeze preservation
radiation injuries
