摘要
通过研究搅拌程序,细胞接种密度,微载体浓度与细胞生长的关系,探索Marc-145细胞在微载体上的生长条件,并测定猪繁殖与呼吸综合征病毒在微载体培养的细胞上的TCID50。结果表明:细胞接种密度为4.28×105 cells/mL时,病毒滴度可达到107.5TCID50,这说明利用微载体繁殖PRRSV是可行的,为猪繁殖与呼吸综合征疫苗的大规模生产奠定实验基础。
The growth condition of Marc-145 cell on the surface of microcarriers was investigated in this experiment by researching on the relationship between the agitation procedure, density of cells inoculated, microcarrier concentration and the growth of Marc-145 cell, and the TCID50 titer of PRRSV was detected also. The result indicated that the viral titer could reach 107.5TCID50 when the density of cells inoculated was 4.28 ×10^5 cells/ml. It was feasible to propagate the porcine reproductive and respiratory syndrome virus on microcarriers, which laid the experimental foundation for mass production of PRRS vaccine.
出处
《广东畜牧兽医科技》
2010年第4期37-40,共4页
Guangdong Journal of Animal and Veterinary Science
基金
广东省农业科技重大专项(2008A020100020)
