摘要
目的:探讨32P-磷酸铬-聚L乳酸(32P-CP-PLLA)粒子植入H22淋巴转移模型的靶向浓聚及对淋巴转移治疗的潜能和间质植入SD大鼠对其免疫功能的影响。方法:KM小鼠模型50只,随机分为2组,瘤体内分别植入1枚32P-CP-PLLA和注入胶体32P-磷酸铬(32P-CP)。给药后不同时间点处死,观察荷瘤鼠体内生物分布及影像学检查,病理学检查。SD大鼠20只,74 MBq/只,γ相机韧致辐射显像,流式细胞仪动态检测血清CD3、CD4、CD8表达水平。结果:体内生物分布示植入32P-CP-PLLA粒子在瘤体内未发生移位。MR扫描示粒子椭圆形低信号区。γ相机韧致辐射显像示植入粒子组放射性分布持续局限浓聚于植入点。SD大鼠肝脏粒子组免疫力3 d开始降低,7 d最低,14 d恢复正常。肌肉粒子组大鼠免疫力14 d略降低,其余时间正常。肝脏32P-CP组免疫力3 d时降低,7 d降到最低,持续30 d恢复正常。结论:32P-CP-PLLA粒子组织靶向定位明显优于32P-CP,对淋巴转移治疗具有一定的潜能。32P-CP-PLLA植入肌肉组织对机体免疫功能无明显影响,植入肝脏免疫功能一过性轻度降低;同等活度的32P-CP肝脏间质注射可引起机体免疫功能明显损伤,持续约4周左右。
OBJECTIVE： To investigate the targeted uptake and potential of lymph metastasis therapeutics by transplanting 32P-chromic phosphate-poly L-lactic acid（32P-CP-PLLA） into H22 lymph metastasis models,and approach the influence of immune function when interstitial implantation was implanted into the SD rats.METHODS： A total of 50 KM mouse models were randomly divided into 2 groups： 32P-CP-PLLA implantation and colloid 32P-chromic phosphate（32P-CP） interstitial injection.In the biodistribution study and pathological examination,mice were sacrificed at different time points after administration,respectively.Imaging examination was performed.Twenty SD rats were administered 74MBq per mice.γ camera imaging was performed then.Different expression levels of CD3,CD4,CD8 in serum were examined by flow cytometer at different time points.RESULTS：The results of biodistribution study revealed that 32P-CP-PLLA seeds remained in transplanted tumors.7.0T Micro MR imaging in mice demonstrated that the seed showed a low oval signal area.γ camera imaging in SD rats demonstrated that in 32P-CP-PLLA group the seeds were limited in the implantation points.In the liver seed group,the immune system of rats appeared impaired at 3 days after administration,went down to a minimum at 7 days and returned to normal at 30 days.In muscle seed group,immunity only appeared a slight fall at 14 days,and maintained normal in the other days.In liver 32P-CP group,the immune system appeared changes at 3 days,went down to a minimum at 7 days,and returned to normal at 30 days.CONCLUSIONS： The targeting positioning can be better of 32P-CP-PLLA than of 32P-CP.The treatment of lymphatic metastasis has some potential.The immune function may have no significant changes through 32P-CP-PLLA seeds implanting in the musculature,while only a slight fall may appear shortly within the liver.Moreover,the 32P-CP at the same dosage can make immune function injury through liver interstitial delivery,lasting around 4 weeks.
出处
《中华肿瘤防治杂志》
CAS
2010年第13期967-971,共5页
Chinese Journal of Cancer Prevention and Treatment
基金
国家高技术研究发展计划(863计划)(2007AA02Z471)
