摘要
目的:探讨CD41标记的微小巨核细胞染色法(SAP染色法)在老年贫血诊断及鉴别诊断中的意义。方法:采用瑞特-姬姆萨染色和CD41标记的SAP染色法,分别检测了107例60岁以上老年贫血患者的骨髓涂片中微小巨核细胞的数量和类型。结果:CD41标记的SAP染色法对巨核细胞的检出率明显高于瑞特-姬姆萨染色法,两者相比差异有统计学意义(P<0.01)。不同疾病组比较,微小巨核细胞阳性率MDS组最高,依次为MDS>ITP>MA>ACD>MM>IDA>RA>AA>PRCA>CAA;淋巴样微小巨核细胞MDS组检出率为73.6%,其他组均未检出。结论:CD41标记的SAP染色可以显著提高巨核细胞检出率;且对老年贫血具有一定的鉴别诊断价值。
Objective：To assess the significance of the CD41 labelled micromegakaryocyte Streptavidin-Alkaline Phosphatase（SAP） staining in the diagnosis and differential diagnosis of senile anemia.Methods：Bone marrow smears from 107 patients with anemia over the age of 60 were detected by Wright-Giemsa staining and CD41 labelled SAP staining for the number and types of micromegakaryocytes,respectively.Results：The detection rates of megakaryocyte by the CD41 labelled SAP staining and Wright-Giemsa staining had significant difference（P 0.01）.Among the groups,MDS group for the positive rate of micromegakaryocyte ranked the first,followed by ITP MA ACD MM IDA RA AA PRCA CAA;Detection rate of lymphoid micromegakaryocyte in the MDS group was 73.6%,there were not micromegakaryocytes observed in other groups.Conclusion：CD41 labelled SAP staining obviously increases the detection rate of megakaryocyte,which is valuable for differential diagnosis of senile anemia.
出处
《东南大学学报（医学版）》
CAS
2010年第4期449-452,共4页
Journal of Southeast University(Medical Science Edition)
关键词
CD41单抗
微小巨核细胞
SAP染色
老年贫血
鉴别诊断
CD41 McAb
micromegakaryocytes
Streptavidin-Alkaline Phosphatase staining
senile anemia
differential diagnosis
