摘要
以红掌盆栽品种‘Avo-Gloria’为试材,以MS+0.2mg·L-12,4-D为基本培养基,分别在添加1～10mg·L-16-BA的10种脱分化培养基上,诱导其叶柄外植体产生愈伤组织;再以MS+2mg·L-16-BA+0.2mg·L-1 NAA为分化培养基诱导分化不定芽;以MS+0.2mg·L-1 NAA为生根培养基,从不定芽获得再生植株。结果显示:(1)在MS+0.2mg·L-12,4-D+8～10mg·L-16-BA的3种脱分化培养基上可产生9%～10%的绿色、质地较硬的愈伤组织;(2)愈伤组织在MS+2mg·L-16-BA+0.2mg·L-1 NAA的分化培养基上,经6～8次继代培养,可获得3%～7%的不定芽,并可生根长成再生植株;(3)再生植株定植3个月后,有3%～7%植株出现红叶变异,此红叶可终生表现为红色。
Leafstalk explants of Anthurium andraeanum pot variety ＇Avo-Gloria＇ were induced to form callus in 10 different de-differentiation mediums.The de-differentiation mediums had MS＋0.2 mg·L^-12,4-D as essential medium with different 6-BA additions,which were ranging from 1 mg·L^-16-BA to 10 mg·L^-16-BA,respectively.Differentiation of adventitious buds was induced in medium of MS＋2 mg·L^-16-BA＋0.2 mg·L^-1NAA,and MS＋ 0.2 mg·L^-1NAA was used as rooting medium to regenerate plants from the adventitious buds.The results showed that the de-differentiation medium with MS＋0.2 mg·L^-12,4-D＋8-10 mg·L^-16-BA could generate 9%-10% green and relatively hard-textured callus.Subcultured 6-8 times on differentiation mediums with MS＋2 mg·L^-16-BA＋0.2 mg·L^-1NAA could get 3%-7% adventitious buds,which could become regenerated plants.After transplanting the regenerated plants for 3 months,3%-7% of the plants have leaf mutation,and the mutated leaves can be red whole life.
出处
《植物生理学通讯》
CSCD
北大核心
2010年第6期571-574,共4页
Plant Physiology Communications
基金
深圳市重点科技计划项目(2003-2-033)
关键词
红掌
组织培养
红叶变异
6-BA
Anthurium andraeanum
tissue culture
leaf mutation
6-BA
