摘要
目的:建立一种检测食品中触酶阳性菌的生物传感法。方法:在一次性丝网印刷电极上聚合辣根过氧化物酶制备检测食品中触酶阳性菌的生物酶传感器,将丝网印刷电极插入电极插口与电化学工作站相连,组装成检测触酶阳性菌的生物电化学传感仪,采用时间-电流法记录响应的结果。结果:对不同来源的食品,当触酶阳性菌菌落数达到104CFU/mL,即可检测到电流变化,检测所需时间根据样品中菌落数的不同而定,一般仅需1～3h即可推测出样品中原有触酶阳性菌含量,最长通过7h的增菌培养即可推测出含有0.1CFU/mL的样品。结论:用该方法检测食品中触酶阳性菌便捷、灵敏、准确,可发展成为一种快速检测食品中触酶阳性菌生物传感器。
Objective： To develop a biosensor for detecting catalase-positive microorganisms in food.Methods： HRP was immobilized onto screen-printed electrodes （SPE） and an analytical system was established by inserting SPE into the electrode slot connected with an electrochemical workstation.Results： For most food samples from different sources,this method allowed detection of positive signals based on current change when the number of catalase-positive microorganism colonies reached up to 104 CFU/mL.The length of time required for food detection was dependent on the number of catalase-positive microorganism colonies and a period of time ranging from 1 to 3 h was enough for the detection of most food samples.Catalase-positive microorganisms contained in food samples at 0.1 CFU/mL needed to be enriched for 7 h,at the longest.Conclusion： This method is convenient,sensitive and accurate,and deserves to be popularized.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2010年第14期174-177,共4页
Food Science
基金
科技部
上海市科委联合资助世博科技专项(06dz05825)
关键词
触酶阳性菌
检测
生物传感器
catalase-positive organism
detection
biosensor
