摘要
为了揭示Pb和NaCl单一及复合胁迫对黄瓜幼苗的伤害机理,采用砂培法,以不添加Pb和NaCl为对照,研究了不同浓度Pb(0.5和1.5 mmol/L)、NaCl(50和100 mmol/L)及其复合胁迫对黄瓜幼苗生理生化特性的影响。结果表明,随着Pb、NaCl处理浓度升高,黄瓜叶片的叶绿素a、叶绿素b和叶绿素a+b含量均显著下降;相对于对照,各处理黄瓜叶片的相对电导率、丙二醛含量明显上升,且100 mmol/L NaCl胁迫下黄瓜叶片的相对电导率和丙二醛含量均高于1.5 mmol/L Pb胁迫。不同处理下的可溶性糖含量、脯氨酸含量、SOD和POD活性均高于对照,且复合处理下的含量高于单一因子处理。NaCl对黄瓜叶片细胞膜的破坏作用强于Pb,Pb、NaCl复合胁迫的毒性效应强于单一胁迫,表现为协同作用。
An experiment was conducted in order to study the injury mechanism of cucumber seedlings under Pb,NaCl and their compound stress. Soil culture experiments were also carried out to study the physiological and biochemical characteristics of cucumber seedlings by applying different levels of Pb（0.5 and 1.5 mmol/L） and NaCl（50 and 100 mmol/L） compound in the soil.The results showed that drastic decrease in the contents of chlorophyll a,chlorophyll b and chlorophyll a＋b of cucumber leaves with increasing Pb and Nacl concentration;In comparison to the control,the relative conductivity and MDA content of cucumber seedlings increased significantly,and ones under 100 mmol/L NaCl treatment were higher than 1.5 mmol/L Pb treatment;The soluble sugar content,proline content,and the activities of SOD and POD increased greatly compared to the control,and ones under compined stress were higher than single stress.Toxic effect of Nacl on the physiological and biochemical characteristics of cucumber was stronger than Pb;and the toxic effect of Pb and Nacl compound stress was always higher than the individual stress.
出处
《干旱地区农业研究》
CSCD
北大核心
2010年第4期85-88,共4页
Agricultural Research in the Arid Areas
基金
河南省重大科技攻关项目(092101310300)
河南省教育厅自然科学研究计划项目(2008B210007)
关键词
黄瓜
PB
NACL
复合胁迫
生理生化特性
cucumber
Pb
NaCl
compound stress
physiological and biochemical characteristics
