摘要
观察白藜芦醇(resveratrol,Res)对胰岛素抵抗大鼠原代脂肪细胞的抗氧化及胰岛素敏感性的影响。培养大鼠原代脂肪细胞,用地塞米松建立胰岛素抵抗模型;添加不同剂量的白藜芦醇,检测葡萄糖的消耗量及培养基和细胞内的超氧化物歧化酶(superoxide dismutase,SOD)、丙二醛(malondialdehyde,MDA)的浓度,Western blot检测沉默信息调节因子1(silent information regulator 1,Sirt1)及胰岛素信号通路相关位点的蛋白表达及相关磷酸化水平。结果显示:Res能增加胰岛素抵抗状态下的脂肪细胞的葡萄糖摄入量,增加SOD,降低脂质过氧化产物MDA;Res增加Sirt1的蛋白表达,经由胰岛素信号转导通路增加葡萄糖转运子4(glucose transporter 4,GLUT4)蛋白的转位。结果说明Res能增加大鼠原代脂肪细胞的抗氧化能力,改善胰岛素抵抗,这可能是通过增加Sirt1表达而实现的。
The effect of resveratrol on the antioxidant status and insulin sensitivity in insulin-resistant rat primary adipocytes was investigated. Rat primary adipocytes were separated and an insulin-resistant model was established using dexamethasone. Glucose consumption as well as SOD and MDA contents in medium and cells treated with resveratrol at varying concentrations was determined. In order to explore the possible mechanisms, the expression and phosphorylation levels of Sirt1, IRS-1, AKT and GLUT4 proteins were examined by western blot. The results indicated that resveratrol increased the glucose consumption and SOD content and suppressed MDA generation in insulin-resistant rat primary adipocytes. Meanwhile, resveratrol also enhanced Sirt1 expression and improved GLUT4 translocation through insulin signaling pathway. Therefore, resveratrol is able to enhance the antioxidant status and insulin resistance in rat primary insulin-resistant adipocytes by exerting its antioxidant activity through Sirt1 pathway.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2010年第13期263-266,共4页
Food Science
基金
广东省医学科研基金资助项目(2008YK0653)
关键词
白藜芦醇
胰岛素抵抗
抗氧化
resveratrol
insulin resistance
antioxidation
