摘要
目的：了解高糖加高胰岛素对人血管平滑肌细胞（ＨＶＳＭＣ）增殖的影响及其有关机制。方法：用含与不含维拉帕米（Ｖｅｒ）的高糖、高胰岛素、高糖加高胰岛素ＤＭＥＭ培养液分别培养人脐动脉平滑肌细胞３５ｄ；并以正常糖（５６ｍｍｏｌ／Ｌ葡萄糖）ＤＭＥＭ培养液培养为对照。结果：高糖、高胰岛素、高糖加高胰岛素培养液培养的各组ＨＶＳＭＣ计数及细胞内游离钙水平均显著高于对照组和含１μｍｏｌＶｅｒ的高糖、高胰岛素、高糖加高胰岛素培养液培养的各组（Ｐ＜００１）。高糖加高胰岛素培养液培养组ＨＶＳＭＣ计数及细胞内游离钙水平显著高于高糖、高胰岛素培养液培养的各组（Ｐ＜００１）。结论：高糖加高胰岛素具有协同促进ＨＶＳＭＣ增殖的作用，这主要是通过增加细胞膜上钙通道开放而实现的。
AIM:To find out that effect of high concentration glucose(Glu)plus high concentration insulin(Ins) on proliferation and free Ca 2+ level in human vascular smooth muscle cells(HVSMCs) and its mechanism.METHOD:Smooth muscle cells from human umbilical artery were cultured in Dulbecco's modified eagle medium (DMEM) with 5.6 mmol/L Glu(control,group 1, n =9),DMEM with 27.8 mmol/L Glu(group 2, n =9),DMEM with 500 mU/L Ins(group 3, n =9),DMEM with 27 8 mmol/L Glu plus 500mU/L Ins(group 4, n =9),DMEM with 1 μmol/L verapamil (Ver)plus 27.8 mmol/L Glu (group 5, n =9),DMEM with l μmol/L Ver plus 500mU/L Ins(group 6, n =9)DMEM with 1 μmol/L Ver plus 27.8 mmol/L Glu and 500 mU/L Ins(group 7, n =9)for 3.5 days.RESULTS:The cell count and free Ca 2+ level of HVSMCs in group 2,3,4 were significantly higher than those of HVSMCs in control and group 5,6,7( P <0.01).The cell count and free Ca 2+ level of HVSMCs in group 4 were significantly higher than those of HVSMCs in group 2,3( P <0.01).CONCLUSION:High concentration glucose plus high concentration insulin coordinately promote proliferation of HVSMCs,and the effect is realized by increasing open of calcium ion channel in membranes.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
1999年第3期237-239,共3页
Chinese Journal of Pathophysiology
关键词
钙
葡萄糖
胰岛素
维拉帕米
糖尿病
血管平滑肌
Proliferation
Calcium
Muscle
smooth
vascular
Glucose
Insulin
Verapamil
