摘要
根据酵母表达系统在表达外源蛋白方面的独特优势,利用酵母表达系统高效分泌并纯化具有生物学活性的蛋白质,已受到广泛关注。信号肽在蛋白质的分泌中起着重要作用,可引导蛋白分泌至胞外,大大提高蛋白的表达量,在工业化生产外源蛋白的纯化工艺方面具有重要意义。该文将从酵母表达系统中对信号肽的选择、改造、偏爱密码子和增强子的应用等几个方面进行优化的探讨,以提高蛋白质在酵母系统中的分泌效率。
Yeasts were used for producing recombinant proteins,because of the unique advantages yeast offered as a unicellular eukaryot.It had become very popular for purified with high biological activity of over-production of both intracellular and extracellular recombinant proteins.Signal peptide played an important role in the secretion of protein and could lead to the extracellular secretion of protein,greatly enhanced the amount of protein expression.It had significance in the industrial production of exogenous protein and the purification.This article reviewed the choice of the signal peptide in the yeast expression system,adaptation,codon preference,the application of Secretion enhancer and sub-optimization of several aspects in order to efficiency improve the protein secretion in the yeast system.
出处
《生物技术》
CAS
CSCD
北大核心
2010年第3期95-97,F0004,共4页
Biotechnology
基金
湖北省自然科学基金项目(2008CDB118)资助
关键词
酵母表达
信号肽
蛋白质
分泌效率
优化
yeast expression
signal peptide
protein
secretion efficiency
optimization
