摘要
为了研究A群链球菌制剂中的活性成分脂磷壁酸(LTA)的提纯方法和抗肿瘤活性,以A群链球菌为出发菌株,采用冷酚法提取A群链球菌制剂中的活性成分脂磷壁酸,经Sepharose CL-4B纯化,用红外图谱比较与脂磷壁酸标品(从金黄色葡萄球菌中提取得到)结构的类同,用气相色谱分析脂磷壁酸中脂肪酸和单糖结构组成,并研究脂磷壁酸的抑瘤活性.结果表明:纯化后的脂磷壁酸所含的蛋白质和核酸污染很低,脂磷壁酸的成分中有甘油磷酸基本骨架、脂肪酸和单糖结构;活性试验证明LTA对子宫癌细胞(Hela细胞)、腹水型EAC瘤和S_180肿瘤具有一定的抑制作用.
To evaluate the antitumor activity of group A streptococcal lipoteichoic acid（LTA）,LTA was extracted and purified by gel permeation chromatography（GPC） from group A Streptococcus,and its molecular structure was analyzed by gas chromatography and infared spectrum.The effect of LTA on the proliferation of human Hela cells and its antitumor activity in vitro were researched.The results showed that LTA was composed of glycerol phosphate backbone,sugar-substituted group and fatty acids.LTA had inhibitory effect on human Hela cells,Ehrlich ascites carcinoma（EAC） and S_180 tumor at a certain dosage range.
出处
《浙江大学学报（农业与生命科学版）》
CAS
CSCD
北大核心
2010年第4期370-374,共5页
Journal of Zhejiang University:Agriculture and Life Sciences
基金
吉林省科技厅计划资助项目(20070930-01)
