摘要
目的 探讨肾母细胞瘤G401与正常胚肾CCC-HEK-1细胞株中微小RNA（miRNA）分子表达谱的差异,为进一步研究相关miRNA在肾母细胞瘤发病机制中的作用奠定基础.方法 选取G401和CCC-HEK-1的各3个样本分别作为实验组和对照组,采用miRNA芯片筛选两者之间差异表达的miRNA,样品间表达变化标准以上调2倍/下调50.0%作为阈值范围,并用实时定量PCR验证miRNA芯片结果的可靠性.结果 上调超过2倍差异表达miRNA分子71个,其中上调超过8倍差异表达miRNA分子11个;其中下调至50.0%以下差异表达miRNA分子59个,其中下调至12.5%以下差异表达miRNA分子11个.总体上,G401和CCC-HEK-1之间差异表达显著.结论 G401和CCC-HEK-1之间存在差异表达miRNA分子,它们可能与肾母细胞瘤的形成有关.
Objective To explore the microRNA（miRNA） differential expression profile between nephroblastoma cell line G401 and normal embryonic kidney cell line CCC-HEK-1 so as to provide rationales for the role of miRNA in the pathogenesis of nephroblastoma. Methods Three samples from G401 cell line and another 3 samples from CCC-HEK-1 cell line were chosen as the experimental group and the control group respectively. miRNA profiles in these samples were analyzed by microarray. The threshold value used to screen up and down-regulated miRNAs was fold change≥2 or≤50.0%. And real-time PCR was used to validate the reliability of microarray. Results Seventy-one miRNAs were found up-regulated in the experimental group and 11 of them were more than 8 times versus control group. In addition, 59 miRNAs were found down-regulated and 11 of them were 12. 5% versus control group. Overall, there were significant differences in the expression of miRNA between G401 and CCC-HEK-1 cell lines. Conclusions There is a differential expression of miRNA between G401 and CCC-HEK-1 cell lines. It may be related with occurrence and metastasis of nephroblastoma.
出处
《中华医学杂志》
CAS
CSCD
北大核心
2010年第26期1845-1848,共4页
National Medical Journal of China
基金
国家自然科学基金（30772273）
河南省医学科技攻关项目（200802004）
