摘要
利用mtDNACyt6基因为标记，研究不同鸽种的亲缘关系和遗传多样性。研究结果：通过对6个鸽种样本Cyt6基因进行PCR扩增和测序，采用生物信息学方法进行数据处理分析，结果显示所有样本中A、G、C、T碱基平均含量分别为26．5％、13．4％、34．8％、25．3％；共发现变异位点数为371个，变异类型为转换和颠换；共发现38个单倍型，平均单倍型多样度（Hd）为0．997，平均核酸多样性（Pi）为0．11373；6个品种之间双参数遗传距离为0．000-0．018，其中王鸽与石岐鸽之间遗传距离最小，岩鸽与蓝环鸽及卡奴鸽间遗传距离最大。结合NCBI上已发表的34个鸠鸽科同源序列进行聚类分析，表明6个家鸽品种只有一个单一的起源。
In order to provide scientific basis for genetic resource protection and utilization of pigeons,genetic diversity and phylogenetic relationships of different pigeon breeds were studied by using mtDNA Cyt b gene as marker,through PCR amplification, sequencing and data analysis by biologic informatics,it showed that A,G,C,T percentages in all samples were 26.5%,13.4%,34.8% and 25.3%,respectively. A total of 371 variation sites were found and all of them were transitions and transversions. In 38 identified haplotype,the average haplotype diversity （Hd） and nueleotide diversity （Pi）was 0.997 and 0.11373 each. The Kimura 2 parameter genetic distance was 0.000 to 0.018 within 6 domestic pigeon breeds,and there was the lowest distance between King pigeon （KiP）and Sheek-kee pigeon （SKP） and the highest one between Hill pigeon（HiP） and either of Blue-ring pigeon（BRP）and Carnean pigeon（CaP）. As far as 34 homologous sequences released by NCBI were used for analysis,we proved that six domestic pigeon breeds had one common ancestor.
出处
《中国家禽》
北大核心
2010年第14期23-26,共4页
China Poultry
基金
广东省教育部产学研结合项目(2007B09040070)
