摘要
目的研究组蛋白乙酰化转移酶亚型类固醇受体共激活因子1(SRC1)在小鼠心脏中的时空表达规律,初步探讨其与心脏发生发育的关系。方法取胎龄E7.5～18、出生1d和3个月成年小鼠正常心脏,每个时间点选9个标本,采用免疫组织化学SP法观察SRC1蛋白在不同发育阶段心脏的空间表达;每个时间点选6组标本,利用蛋白质印迹分析技术检测其在小鼠心脏发育过程中时序性表达变化。结果免疫组织化学结果显示,SRC1蛋白在E7.5胚胎心脏原基中不表达;E8.5～E9.5心管中微弱表达;E10.5后心脏发育各时期,小梁弱表达,心脏其他区域均有较强而广泛表达。蛋白质印迹分析结果显示,E10.5以后的心脏发育阶段,SRC1蛋白在E10.5较低表达,与E11.5、E12.5相比表达差异有统计学意义(P<0.05),与其他发育时间点相比表达差异无统计学意义(P>0.05);E11.5～E12.5达到表达高峰,此两个发育时间点相比表达差异无统计学意义(P>0.05),与其他发育时间点相比表达差异有统计学意义(P<0.05);此后表达下降并持续低表达至成年鼠期,此阶段表达差异无统计学意义(P>0.05)。结论 SRC1蛋白广泛表达于E8.5后的发育心脏,并呈时空动态变化,提示其参与了心脏发育的整体调控,可能与心脏间隔早期的诱导形成关系更为密切。
Objective To study the spatiotemporal expression pattern of histone acetyltransferase steroid receptor coactivator-1（SRC1）in developing mouse heart,so as to explore the relationship of SRC1 with heart development.Methods The normal mouse hearts were collected at E7.5~E18 and 1 day and 3 months after birth;9 specimens were chosen for each time point to observe the spatial expression of SRC1 by immunohistochemistry technique,and 6 specimens of each time point were used to examine the temporal expression of SRC1 protein by Western blotting technique and to plot the time-dose curve.Results Immunohistochemistry showed no expression of SRC1 in the heart primordium at E7.5;only very weak SRC1 expression was found in the cardiac tube at E8.5~E9.5.Weak SRC1 expression was found in the trabeculae after E10.5 and relatively strong and widespread expression was found in other heart regions.Western blotting results demonstrated that SRC1 protein expression at E10.5 was significantly lower than those at E11.5 and E12.5（P0.05）,and was not significantly different with those of other time points after E10.5（P0.05）.SRC1 expression reached the peak at E11.5~E12.5,and there was no significant difference between the two time points（P0.05）.SRC1 expression gradually decreased after E11.5~E12.5 till the adulthood,and there were no significant differences in the expression after E13.5（P0.05）.Conclusion Widespread distribution of SRC1 is present in the developing mouse heart after E8.5,and the expression is in a dynamical spatiotemporal pattern,suggesting that SRC1 may take part in the overall regulation of the heart development,and it might has a closer relationship with the early induction of the heart septa.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
2010年第7期744-747,共4页
Academic Journal of Second Military Medical University
基金
国家自然科学基金(30770889)
高等学校博士学科点专项科研基金(20060631004)~~
