摘要
目的 观察血管紧张素Ⅱ（AngⅡ）对急性肺损伤（ALI）大鼠肺水通道蛋白1（AQP1）表达的影响及在肺水肿形成中的作用.方法 按随机数字表法将40只SD大鼠分为假手术组、模型组、AngⅡ受体阻滞剂预处理组及治疗组,每组10只.采用失血性休克-内毒素二次打击建立大鼠ALI模型.预处理组静脉注射脂多糖（LPS）前30 min注射AngⅡ受体阻滞剂30 μg/kg,注射LPS后30 min注射30 μg/kg生理盐水;治疗组注射LPS前30 min注射30 μg/kg生理盐水,注射LPS后30 min再注射AngⅡ受体阻滞剂30 μg/kg;模型组注射LPS前、后均给予30 μg/kg生理盐水.制模后6 h处死大鼠,取下腔静脉血,用放射免疫法测定血清肿瘤坏死因子-α（TNF-α）水平;取肺组织,计算湿/干重（W/D）比值,用放射免疫法测定肺组织AngⅡ表达,用逆转录-聚合酶链反应测定AQP1 mRNA表达.结果 与假手术组相比,ALI大鼠血清TNF-α水平及肺组织W/D比值、AngⅡ表达明显增加,AQP1 mRNA表达明显减少.预处理组及治疗组血清TNF-α水平（μg/L）较模型组明显减少（4.79±0.24、5.55±0.36比6.34±0.31,均P〈0.05）,肺组织W/D比值减小（4.34±0.23、4.85±0.20比5.41±0.26,均P〈0.05）,AQP1 mRNA表达明显增加（0.854±0.067、0.727±0.081比0.358±0.071,均P〈0.05）;而AngⅡ表达（ng/g）有所降低（172.19±15.82、202.82±20.47比245.88±26.31）,但差异无统计学意义（均P〉0.05）.AQP1 mRNA表达与AngⅡ表达和肺组织W/D比值均呈负相关（r1=-0.782,r2=-0.726,均P〈0.05）.结论 ALI时AngⅡ可能直接或通过炎症介质下调肺脏AQP1 mRNA表达,为肺水肿形成的机制之一.
Objective To investigate the effect of angiotensinⅡ （AngⅡ） on the expression of aquaporin 1 （AQP1） in lung of rats with acute lung injury （ALI） and the role of AngⅡ in the formation of lung edema. Methods Forty healthy Sprague-Dawley （SD） rats were randomly divided into sham-operated group, model group, AngⅡ receptor blocker pretreatment group, and AngⅡ receptor blocker treatment group according to random digits table, with 10 rats in each group. ALI model of rats was reproduced with administration of endotoxin after hemorrhagic shock. In rats of pretreatment group AngⅡ receptor blocker 30 μg/kg was given 30 minutes before lipopolysaccharide （LPS） injection; rats in treatment group AngⅡ receptor blocker 30 μg/kg was given 30 minutes after LPS injection; rats in model group received 30 μg/kg normal saline 30 minutes before and after LPS injection. Rats were sacrificed 6 hours after model establishment, samples of venous blood and lung tissue were collected, radioimmunoassay was used to measure the level of tumor necrosis factor-α （TNF-α） in serum and the expression of AngⅡ in lung tissue, ratio of wet to dry （W/D） weight of lung tissue was calculated, reverse transcription-polymerase chain reaction was used to measure the expression of AQP1 mRNA in lung tissue. Results Compared with rats of sham-operated group, the level of TNF-α in venous blood, W/D ratio and the expression of AngⅡ in lung tissue increased significantly, the expression of AQP1 mRNA in lung tissue decreased significantly in rats of ALI. Compared with rats of model group, the level of TNF-α in venous blood （μg/L） decreased significantly （4.79±0.24, 5.55±0.36 vs. 6.34±0.31, both P〈0.05）, W/D ratio decreased significantly （4.34±0.23, 4.85±0.20 vs. 5.41±0.26, both P〈0.05）, the expression of AQP1 mRNA in lung tissue increased significantly （0.854±0.067, 0.727±0.081 vs. 0.358±0.071, both P〈0.05）, and the expression of AngⅡ in lung tissue （ng/g） decreased to some e
出处
《中国危重病急救医学》
CAS
CSCD
北大核心
2010年第7期426-429,共4页
Chinese Critical Care Medicine
基金
江西省医药卫生科技计划基金项目（20073031）
