摘要
目的 观察嗜酸乳杆菌（L.acidophilus）对乳鼠感染人轮状病毒（HRV）的可能预防和治疗作用.方法 将60只昆明种乳鼠随母鼠按窝随机均分为4组,给4 d龄的乳鼠灌服HRV作为HRV感染组,灌服HRV的前和后灌服嗜酸乳杆菌作为嗜酸乳杆菌预处理组和治疗组,正常对照组灌服同剂量最低限度必要成分（MEM）Eagle细胞培养液.观察各组乳鼠的临床症状、肠黏膜病理变化;酶联免疫吸附法检测各组乳鼠粪便HRV抗原;免疫荧光法检测HRV抗原在各组乳鼠小肠组织中的表达.结果 对照组乳鼠未出现腹泻.预处理组和治疗组乳鼠的腹泻程度、腹泻持续天数、HRV阳性天数和死亡率均低于HRV感染组;其肠黏膜的病变程度及HRV抗原表达也明显弱于HRV感染组.结论 嗜酸乳杆菌对乳鼠感染人轮状病毒有预防和治疗作用.
Objective To investigate the potential effect of Lactobacillus acidophilus （L. acidophilus） on prevention and treatment of neonatal mice infected with human rotavirus （HRV）. Methods Sixty 4-day-old kunming mice were randomly divided into control group. HRV infected group, L. acidophilus pretreated group （treated before HRV infection ） and L. acidophilus treated group（treated after HRV infection）. The manifestation and pathological changes in small intestine of neonatal mice were observed. The HRV antigen in the feces and intestines were measured by ELISA and fluorescent-focus assay, respectively. Results The severity and duration of diarrhea as well as mortality in L. acidophilus pretreated group and treated group were lower than those in HRV infected group. The duration of HRV-antigens shedding following infection was considerably prolonged in HRV infected group compared to that in L. acidophilus pretreated group and treated group. Furthermore, decreased expression of HRV antigen and little pathological changes in intestinal mucosa were found in L. acidophilus pretreated group and treated group when compared with HRV infected group. Conclusion L. acidophilus may be used as an alternative approach for the prevention and treatment of neonatal mice infected with HRV.
出处
《中华消化杂志》
CAS
CSCD
北大核心
2010年第7期465-469,共5页
Chinese Journal of Digestion
关键词
轮状病毒
嗜酸乳杆菌
乳鼠
腹泻
肠黏膜
Rotavirus
Lactobacillus acidophilus
Neonatal mice
Diarrhea
Intestinal mucosa
