摘要
目的分别建立免疫磁珠分离和RT-PCR技术检测胃癌患者外周血中微转移的情况,讨论两种检测方法的意义和差别以及微转移与临床病理的相关性。方法首先采用密度梯度离心的方法分离出胃癌和慢性胃炎患者外周血中的单个核细胞,再分别采用RT-PCR和免疫磁珠技术检测其中的癌细胞。并行HE染色、免疫细胞化学和免疫荧光化学的方法对于获得的细胞进行检测分析。结果 60例胃癌患者有24例采用免疫磁珠分选技术分离到微转移的癌细胞,而RT-PCR法阳性者为32例。慢性胃炎患者的检测结果均为阴性。微转移的检出与胃癌组织的分化程度呈正相关。结论胃癌患者的外周血中存在着微转移的癌细胞,采用免疫磁珠分离技术和RT-PCR的方法均可检测到微转移的癌细胞。RT-PCR法的敏感性高于免疫磁珠分离技术,更适合于临床应用。高分化的胃癌更易发生血行转移。
Objective To discuss the significance and difference of immunomagnetic beads sorting technique and RT-PCR technique and the relationship between the pathological classification of primary focus and the positive rate of micrometastasis by detecting the micrometastasis of gastric carcinoma in peripheral blood circulation by using these two methods. Methods Density gradient centrifugation was used to isolate mononuclear cells from peripheral blood, immunomagnetic beads sorting technique and RT-PCR technique were used to detect the disseminated carcinoma cells. HE, immunocytochemical and immunofluorescence staining were also used to identify the characteristics of the separated cells. Results Carcinoma cells were found in 24 of 60 specimens of peripheral blood by immunomagnetic beads sorting technique while 32 positive cases were found by RT-PCR technique.＂ All the patients with chronic gastritis were negative. There was a significant difference between the positive rates of two methods in detecting the disseminated carcinoma cells from the peripheral blood circulation of gastric carcinoma patients. The positive detection was significantly correlated with the pathological classification of primary focus. Conclusion There are disseminated carcinoma cells in the peripheral blood circulation in patients with gastric carcinoma. Disseminated carcinoma cells can be detected from the peripheral blood samples with immunomagnetic beads sorting technique and RT-PCR technique. The positive rate of RT-PCR technique is higher than that of immunomagnetic beads sorting technique in detecting micrometastasis. The positive rate of micrometastasis of peripheral blood circulation is correlated with the pathological classification of primary focus.
出处
《同济大学学报（医学版）》
CAS
2010年第3期45-49,共5页
Journal of Tongji University(Medical Science)
关键词
胃肿瘤
微转移
免疫磁珠
聚合酶链反应
gastric carcinoma
micrometastasis
immunomagnetic beads
RT-PCR
