摘要
目的研究转化生长因子β１（ＴＧＦβ１）ｍＲＮＡ在原发性肝细胞癌患者中的表达及其临床意义。方法用ＲＴ－ＰＣＲ加ＤｏｔＢｌｏｔ法检测原发性肝细胞癌患者肝组织和外周血单个核细胞（ＰＢＭＣ）中ＴＧＦ－β１ｍＲＮＡ水平，并以正常肝组织和正常人的ＰＢＭＣ为对照。结果ＴＧＦ－β１ｍＲＮＡ水平在原发性肝细胞癌患者组肝组织（２．２２±０．８４，ｎ＝１６）和ＰＢＭＣ中（１．８３±１．２，ｎ＝２５）比较，差异无显著性（Ｐ＞０．０５），但两者均高于正常肝组织（０．９４±０．７６，ｎ＝５）和正常人的ＰＢＭＣ（０．６２±０．４０，ｎ＝１６）水平。结论ＴＧＦ－β１ｍＲＮＡ水平与原发性肝细胞癌有关，ＰＢＭＣ中ＴＧＦ－β１ｍＲＮＡ检测可望作为一项代替肝组织活检的指标，其表达水平与肝癌有关。
Objective To examine the expression of transforming growth factor β 1(TGF-β 1) in peripheral blood mononuclear cells(PBMC) in patients with hepatocellular carcinoma(HCC) and study its clinical value.Method Reverse transcription polymerase chain reaction add Dot blot analysis was used to examine TGF-β 1 mRNA levels of liver tissue and PBMC in patients with HCC.Result There was no significant difference between PBMC(1.83±1.20, n=25) and liver tissue(2.22±0.84,n=16) in patients with HCC( P <0.05), and they were all higher than those of normal (0.62±0.40,n=16, and 0.94±0.76, n=5).Conclusion The level of TGF-β 1 mRNA is related with HCC, and the measurement of GTF-β 1 mRNA in PBMC may substitute the measurement in liver biopsy.
出处
《中华消化杂志》
CAS
CSCD
北大核心
1999年第1期29-31,共3页
Chinese Journal of Digestion
