摘要
目的:建立复方神安颗粒中苦参碱含量测定方法。方法:采用高效液相色谱法,Dikma Diamonsil(钻石)C18色谱柱(4.6 mm×150 mm,5μm);流动相乙腈-0.1%磷酸水(22∶78)(三乙胺调到pH7.5);流速1 mL.min-1;检测波长220 nm;柱温25℃;进样量10μL。结果:苦参碱在2.08～10.40μg(r=0.999 6)呈良好的线性关系,平均回收率为100.51%(RSD2.0%)。结论:本方法操作简便,准确,重复性好,精密度高,可作为该制剂的质量控制方法。
Objective： To determination the content of matrine in Fufang Shen′an particles by HPLC.Method： HPLC with Dikma Diamonsil C18（4.6 mm×150 mm,5 μm） column was used.Acetonitrile-0.1%H3PO4（22∶78）as the mobile phase,flow rate was 1.0 mL·min-1 and the UV detection wavelength was 220 nm.Result： The linear range of matrine was 2.08-10.40 μg,the correlation coefficient（r） was 0.999 6.The average recovery rate was 100.51%,RSD was 2.04%（n=6）.Conclusion： The method is suitable for the formulate of the quality standard with sufficient accuracy,stability and reappearance.It can be used for quality control in Fufang Shen′an particles.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2010年第14期1818-1819,共2页
China Journal of Chinese Materia Medica
