摘要
目的 观察Toll样受体4（TLR4）在人胆囊癌细胞SGC-996的表达及RNA干扰TLR4基因表达后对裸鼠体内成瘤能力的影响.方法 采用免疫组化及逆转录.聚合酶链反应（RT-PCR）检测TLR4在SGC-996细胞的表达.将12只BALB/C裸鼠随机分为两组,实验组皮下注射6×106个经TLR4小干扰RNA稳定转染后的SGC996细胞,对照组注射同数量未经处理的SGC996细胞,连续观察5周,比较两组肿瘤的生长情况,计算成瘤率、生长体积和生长率.结果 SGC-996细胞阳性表达TLR4.两组裸鼠成瘤率均为100%,瘤体平均体积分别为（277.94±7.60）mm3和（474.47±9.99）mm3,平均生长率分别为7.941%和13.556%,瘤体质量分别为（0.76±0.16）g和（1.78±0.23）g,差异有统计学意义（P〈0.05）.结论 TLR4表达于人胆囊癌细胞SGC-996,小干扰RNA转染后可以降低TLR4基因表达,从而使SGC996细胞体内成瘤能力下降.
Objective To observe the expression of TLR4 in human gallbladder cancer cell line SGC-996,and investigate whether RNA interference targeting TLR4 gene affect growth of the gallbladder carcinoma xenograft in nude mice. Methods The expression of TLR4 in SGC-996 was determined bv im-munohistochemistry and reverse transcription-polymerase chain reaction （ RT-PCR）. 12 BALA/C nude mice were randomly divided into two groups. The experimental group was injected 6 x 10 SGC996 cells stable transfected by small interfering RNA for TLR4 per mice. The control group was injected with the same number of untreated SGC996 cells. Aftter continuous observation for 5 weeks, the formation rate, growth rate, weight and volume of the tumors were measured and calculated. Results The results of immunohistochem-istry and RT-PCR indicated the expression of TLR4 in SGC-996. The tumor formation rate of gallbladder carcinoma cell and TLR4-siRNA transfected SGC996 cell was 100%. The average tumor volume in experimental group and control group was （277.94±7.6 ） mm3 and （474.47±9.99） mm3 respectively. The growth rate was 7. 941% and 13. 556% ,and the average tumor weight was （0.76 ±0.16） g and （1.78± 0.23） g,respectively. The tumor growth rate,weight and volume in the experimental group decreased significantly when compared with those in control group （P〈0.05）. Conclusion Human gallbladder carcinoma cell line SGC-996 expresses TLR4. SGC-996 cells transfected with siRNA can effectively lower the level of TLR4 gene expression. After silence of TLR4 gene,the growth rate,weight and volume of xenograft are decreased significantly.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2010年第7期860-862,共3页
Chinese Journal of Experimental Surgery
关键词
TLR4
胆囊癌
RNA干扰
裸鼠
TLR4 Gallbladder carcinoma RNA interference Nude mice
