摘要
目的:观察桂枝麻黄各半汤、银翘散、新加香薷饮对甲1、甲3型流感小鼠白细胞介素2(IL-2)、T淋巴细胞亚群的影响。方法:选取SPF级NIH小鼠,毒种流水冲浸融化,小鼠经乙醚吸入麻醉后,病毒滴鼻每只约0.05mL。在病毒攻击前1d开始给药,以3种解表方中药组分别按最适合有效剂量(0.4mL·d-1)ig给药5d。至造模后第4d,球后取血,测IL-2和T淋巴细胞亚群CD3+,CD4+,CD8+。结果:感染甲1、甲3型流感病毒后,外周血IL-2的浓度明显降低,与正常组比较,差异有统计学意义(P<0.05)。银翘散对甲3型流感感染小鼠IL-2升高无统计学意义,病毒唑组及3种解表方法均能提高病毒感染小鼠外周血IL-2水平,且在甲1型实验中,中药治疗各组均高于正常对照组水平,差异有统计学意义(P<0.05)。感染甲1型流感病毒后,小鼠外周血中T淋巴细胞CD3+,CD4+降低,与正常对照组比较,差异有统计学意义(P<0.05),尤其CD4+降低明显,致CD4+/CD8+比例下降,与正常对照组比较,差异有统计学意义(P<0.05),提示细胞免疫功能下降失调。而3种解表方法中药均能提高CD3+,CD4+总数,与模型对照组比较差异有显著性意义(P<0.05),3治疗组CD8+也较正常组高(P<0.05),但3组的CD4+/CD8+比值较模型对照组高(P<0.05),已较接近正常水平。结论:3种解表方(桂枝麻黄各半汤、银翘散和新加香薷饮)均能不同程度地提高机体细胞免疫功能,达到抗流感病毒的效果。
Objective：To observe the effect of Guizhi Mahuang Geban Tang（GMGT,Integrated Decoction of Ramulus Cinnamomi Decoction and Herba Ephedrae Decoction） ,Yinqiao San（YS,Powder of Flos Lonicerae and Fructus Forsythiae） ,and Xinjia Xiangru Yin（XXY,Modified Herba Moslae Powder） on interleukin 2（IL-2） and T lymphocyte subgroups in mice infected with influenza A virus of type 1 or type 3. Method：NIH mice with specific pathogen free were given GMGT,YS and XXY（0. 4mL · d^-1,ig） respectively one day before the mice were attacked by intranasal dripping of 0. 05 mL influenza A virus solution. After establishing the model,blood sample was collected on the fourth day,and the amount of IL-2 and CD 3^＋,CD 4^＋,CD 8^＋ in the blood was examined. Result：After infection,the peripheral IL-2 level in infected mice was decreased as compared with the normal group（P〈 0. 05） . YS had no significant effect on mice infected with type 3 influenza A virus. Both virazole and three exterior-releasing therapies increased the peripheral IL-2 level in mice infected with influenza A virus,and three exterior-releasing therapies increased the peripheral IL-2 level in mice infected with type 1 influenza A virus（P〈 0. 05,compared with the normal group） . CD 3^＋ and CD 4^＋ percentage in the mice infected with type 1 influenza A virus decreased as compared with those in the normal group（P〈 0. 05） ,and the decrease of CD 4^＋ percentage in the infected mice was obvious,resulting in the lower CD 4^＋ /CD 8^＋（P〈 0. 05 compared with the normal group） . Three exterior-releasing therapies increased the total amount of CD 3^＋ and CD 4^＋（P〈 0. 05,compared with the model group） ,and increased the CD 8^＋（P〈 0. 05,compared with the normal group） and CD 4^＋ /CD 8^＋（P 〈0. 05,compared with the model group） ,being close to the normal level. Conclusion：The anti-virus mechanism of the three exterior-releasing therapies,GMGT,YS and XXY,is probably related with elevation of the cellu
出处
《中国实验方剂学杂志》
CAS
北大核心
2010年第7期108-111,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家中医药管理局基金项目(04-05JP46)
国家自然科学基金项目(39700184)
关键词
流感
病毒
中药组方
白介素-2
T淋巴细胞亚群
influenza
virus
Chinese herbal prescriptions
interleukin 2
T lymphocyte subgroups
