摘要
目的探讨五价砷和三价砷的细胞转化活性的异同。方法应用细胞转化试验和软琼脂培养技术，以ＮＩＨ３Ｔ３为靶细胞。结果在所设剂量下，两种受试物均可诱发细胞转化，并有一定的剂量依存趋势。两种受试物比较，亚砷酸钠的致细胞转化作用较砷酸钠强。软琼脂试验显示，两种受试物的各个剂量，均诱发了阳性反应，与细胞转化试验结果相一致。结论因进入体内的五价砷可以还原成三价，对工业生产中接触的五价砷的致癌危险性不容忽视。
Objective To investigate the difference of cell transformation activity between pentavalent arsenate and trivalent arsenite.Methods Cell morphological transformation test and soft agar assay were used,and NIH3T3 cell line was chosen as the target cell.Results Showed that the two compounds exhibited positive effects at the designed doses in a dose dependent manner,the trivalent arsenate was more potent than that of the pentavalent arsenite.The positive result of the two compounds in soft agar assay showed a consistency with that seen in the transformation test.Conclusion Seems that as the pentavalent arsenic can be reduced to trivalent arsenic in human body,it should not ignore the risk of exposure to pentavalent arsenic in working environment.
出处
《中国工业医学杂志》
CAS
1999年第1期10-12,共3页
Chinese Journal of Industrial Medicine
关键词
砷酸钠
亚砷酸钠
细胞转化
软琼脂试验
致癌性
Sodium arsenate,Sodium arsenite,Cell transformation,Soft agar assay
