摘要
目的分析鲍氏不动杆菌β-内酰胺酶基因型及同源性,了解其流行情况。方法采用PCR法检测β-内酰胺酶基因型并经DNA测序确证,用重复片段引物PCR判定基因的同源性。结果对46株鲍氏不动杆菌的耐药基因检测发现,单纯TEM阳性25株,占54.35%;单纯PER阳性5株,占10.87%;另6株(13.04%)同时含有TEM和PER基因;重复片段引物PCR显示,46株鲍氏不动杆菌基因为1～5条大小<2000 bp的片段,共分为20个基因型,其中A型16株(34.78%),C型和E型各4株(8.70%),J型3株(6.52%),B、K、H型各2株(4.35%),其余类型各1株(2.17%)。结论鲍氏不动杆菌基因型以TEM型为主,重复片段引物PCR显示A型菌株为主要的流行株。
OBJECTIVE To investigate the genotypes and genetic homology of beta-lactamases of Acinetobacter baumannii and evaluate the prevalence of A.baumannii.METHODS The main genotypes of beta-lactamases were detected by PCR and confirmed by DNA sequence test.The genetic identity was determined by the repetitive extragenic palindromic sequence-based PCR（REP-PCR）.RESULTS Among 46 isolates of A.baumannii,TEM type of beta-lactamases was found in 25 isolates,PER-positives were observed in 5 isolates.Six isolates harbored both TEM and PER genes.The products of REP-PCR from 46 isolates were separated into 1-5 bands with size 〈2000bp fragments,and divided into 20 genotypes.Among the 20 genotypes of the isolates,16（34.78%） isolates belonged to type A,4（8.70%） isolates belonged to type C and 4 to type E,3（6.52%） isolates belonged to type J,each 2（4.35%） in 6 isolates belonged to types J,B and K,respectively,and each 1（2.17%）in 13 strains belonged to other different types.CONCLUSION TEM type of beta-lactamase is the main genotype of A.baumannii.Among 20 genotypes showed in REP-PCR,type A is the main genotype of A.baumannii.
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2010年第13期1837-1839,共3页
Chinese Journal of Nosocomiology
基金
广西科学基金资助项目(桂科自0447052)
关键词
鲍氏不动杆菌
Β-内酰胺酶
重复序列聚合酶链反应
基因同源性
Acinetobacter baumannnii
Beta-lactamase
Repetitive extragenic palindromic sequence-based PCR（REP-PCR）
Genetic homology
