摘要
应用愈伤组织浸渍处理将甘蔗近缘植物竹、芒和割手密的DNA导入甘蔗栽培种中,结果在不同组合出现了许多甘蔗常规组培过程中未曾发现的变异。从5个组合中选育出11个稳定遗传的变异系,其叶圆片经PEG渗透胁迫后的抗氧化代谢分析结果表明,外源DNA导入甘蔗变异系的抗氧化代谢反应基本介于DNA供体和受体之间,竹、芒、割手密DNA处理选育的变异系均不同程度地提高了抗渗透胁迫能力,尤其是芒DNA处理效果优于割手密和竹,表现为较高的SOD、CAT活性和GSH含量以及较低的MDA含量和质膜透性,最后还对DNA导入后代产生变异的机理进行了初步分析。
DNA of the sugarcane (Saccharum officinarum L. ) relative Sinocalamus oldami (Munro) Mucculure, Miscanthus floridulm (Labill) Warb and Saccharum spantaneum L. was introduced into sugarcane cultivars Funong 79-23 and Funong 83-0706 through macerative absorption of calli. A lot of variations that were never found in the native tissue culture occured in the progenies of receptors. Leaf discs of eleven good variation lines with stabilized heredity from 5 combinations were analyzed for anti-oxidation metabolism under PEG osmotic stress. The characters of the anti-oxidation metabolism in these variation lines were mostly between those of the DNA receptors and donors. The ability of anti-osmotic stress of the DNA receptors was raised by introducing the above relative DNA into them, especially by Miscanthus flaridulus (Labill) Warb DNA, which showed the higher SOD, CAT activities and more GSH content, as well as the lower MDA content and plasma membrane permeability. The mechanism for variation of the receptor causd by exogenous DNA were primarily discussed in this paper.
出处
《作物学报》
CAS
CSCD
北大核心
1999年第1期8-15,共8页
Acta Agronomica Sinica
关键词
甘蔗
外源DNA
抗氧化代谢
渗透胁迫
Sugarcane (Saccharum officinarum L. )
Exogenous DNA
Anti-oxidation metabolism
Osmotic stress
