摘要
目的：探讨CD88在肝细胞肝癌中的表达及其与临床病理特征的关系。方法：应用实时荧光定量聚合酶链反应（qRT-PCR）、免疫组织化学和Western blot检测56例肝癌及癌旁组织标本中CD88的表达,比较CD88表达在癌和癌旁组织中的差别,并分析其与临床病理特征的关系。结果：qRT-PCR检测结果显示肝癌组织中CD88mRNA的表达明显高于癌旁组织（0.056±0.014比0.003±0.0004,P〈0.001）;癌组织中CD88mRNA表达在肿瘤直径大于5cm、有肉眼癌栓和TNM分期为Ⅲ~Ⅳ期的肝癌患者中明显上调,分别为0.1119±0.0339比0.0256±0.0094、0.0907±0.0271比0.0245±0.009、0.0876±0.02630比0.0253±0.0096（P〈0.05）;免疫组织化学检测结果显示肝癌CD88阳性率明显高于癌旁组织（55.3%比37.5%,P〈0.05）;Western blot检测结果显示肝癌组织中CD88表达高于癌旁组织（3.71±0.92比0.91±0.34,P〈0.01）。结论：CD88高表达与肿瘤的恶性表型密切相关,它可能在肝癌侵袭和转移过程中发挥重要作用。
Objective：To investigate the expression of CD88 in hepatocellular carcinoma（HCC） and its clinical significance.Methods： The different expression of CD88 mRNA and protein levels in HCC and peritumoral tissues in 56 HCC patients were determined by qRT-PCR,immunohistochemistry and Western blot,respectively.The clinical significance of CD88 was further explored.Results： The CD88 mRNA expression was significantly higher in HCC tissues than that in the perfumoral tissues（0.056±0.014 vs.0.003±0.0004,P0.001）.Further more,the expression of CD88 mRNA in HCC tissues was higher in HCC patients with tumor size over 5 cm（0.1119±0.0339 vs.0.0256±0.0094）,with tumor thrombus（0.0907±0.0271 vs.0.0245±0.009）,and with TNM stage Ⅲ~Ⅳ（0.0876±0.02630 vs.0.0253±0.0096）（all P0.05）.Immunohistochemistry results showed that CD88 positive rates in liver cancer and its adjacent tissues were 55.3% vs.37.5%（P0.05）,Western blot results also showed that expression of CD88 in tumor tissue was higher than in adjacent noncancerous tissues（3.71 ±0.92 vs.0.91 ±0.34,P0.01）.Conclusions： The over-expression of CD88 is associated with HCC malignant phenotype;CD88 may play an important role in HCC invasion and metastasis.
出处
《中国临床医学》
2010年第3期328-330,共3页
Chinese Journal of Clinical Medicine
基金
国家自然科学基金(编号:308730393)
