摘要
利用3个真菌菌株ZB-4、ZW-3、XX-8的发酵液对蕃茄根结线虫2龄幼虫进行室内离体活性测定和温室盆栽的防效试验。结果表明:供试3个菌株不同稀释倍数的发酵液在处理后24、48和72 h对番茄根结线虫都有一定的抑制作用,其中3个菌株的2倍发酵液在处理后72 h对番茄根结线虫2龄幼虫的校正抑制率分别为95.27%、91.27%、86.58%;4倍发酵液在处理后72 h对番茄根结线虫2龄幼虫的校正抑制率分别为68.52%、84.05%、60.33%。在温室盆栽试验中,用3个菌株的2倍发酵液处理番茄苗,与对照相比,处理后45 d番茄根结线虫的减退率分别达到46.7%、43.3%、36.7%,防治效果分别达到23.81%、19.52%、35.71%。此外,处理后22 d和45 d的调查结果显示,3个菌株的2倍发酵液对番茄生长有一定的促进作用。
The three fungal fermentation liquid of ZB-4,ZW-3 and XX-8 were used in treating vegetable root-knot nematodes in vitro bioassay and in the greenhouse pot experiment.The results showed that： in 24,48,72 hours treatment,all the fermentation liquid of ZB-4,ZW-3 and XX-8 have certain inhibitory effect on root-knot nematodes at different dilution ratios.In 72 hours treatment,the 2 times dilution of the three fungi strains fermentation liquid broth showed the rectified inhibiting efficiencies to J2 as 95.27%,91.27% and 86.58% respectively.The rectified inhibiting efficiencies to J2 of 4 times dilution were 68.52%,84.05% and 60.33% in 72 hours.In greenhouse pot experiment,the 2 times broth of three fungi were used to treat tomato seedlings.After 45 days,compared to the control,the reducing rate of nematodes were up to 46.7%,43.3% and 36.7% respectively.The controlling efficiencies reached 23.81%,19.52% and 35.71% respectively.Furthermore,our investigation in 22 days and 45 days showed that the 2 times broth could promote the growth of tomatoes.
出处
《华中农业大学学报》
CAS
CSCD
北大核心
2010年第4期440-443,共4页
Journal of Huazhong Agricultural University
基金
国家公益性行业科研专项(nyhyzx07-050)
北京市农委科技服务项目(BJNY2007-03)资助
关键词
蕃茄根结线虫
生物防治
真菌发酵液
tomato root-knot nematode
biological control
fungal fermentation liquid
