摘要
目的:本实验通过体外培养人Burkitt’s淋巴瘤Raji细胞,观察选择性COX-2抑制剂Celecoxib对Raji细胞增殖、凋亡及细胞周期分布的影响。方法:体外培养人Burkitt’s淋巴瘤Raji细胞,用不同浓度的Cele-coxib进行干预。应用MTT比色法检测细胞生长情况;流式细胞术(FCM)分析Celecoxib对细胞周期的影响并检测细胞凋亡的情况。结果:Celecoxib各浓度组作用于Raji细胞24、48、72h后吸光度值与对照组相比均有显著性差异(P<0.05),同时各浓度组之间以及时间组之间有显著性差异(P<0.05).Celecoxib各浓度组作用于Raji细胞48h后出现明显的凋亡峰,各实验组与对照组相比均具有显著差异(P<0.05),细胞周期分布也发生明显变化(P<0.05)。各浓度组间Celecoxib对细胞凋亡率和细胞周期的影响有显著差异(P<0.05)。结论:Celecoxib体外对Raji淋巴瘤细胞生长具有明显的抑制作用。通过阻滞细胞周期于GO/Gl期,抑制Raji细胞的增殖,此可能为非COX-2依赖性途径作用机制之一。
Objective： To investigate the possible antitumor mechanism of Celecoxib in COX-2 independent pathway, and to observe the effect of selective COX-2 inhibitor Celecoxib on proliferation, apoptosis, cell cycle distribution in human malignant lymphoma cell line Raji. Methods ： Human Burkitt＇ s lymphoma cells Raji were cultivated in vitro and then treated with various concentrations of Celecoxib. Cell inhibitive rate was investigated by MTT assay. Apoptosis rate and cell cycle distribution were investigated by FCM. Results： Absorbance light degree in experiment groups after treated Raji cells for 24,48,72 h were significantly different compared with control group （P 〈 0.05）. At the same time, there was a significant difference between concentration groups and time groups （P 〈 0.05）. Apoptosis peak emerged significantly after Raji cells treated with Celecoxib for 48 hours. AP were significant higher in experiment groups than that in control group and AP were significantly different among concentration groups （P 〈 0.05 ）. Compared with control group, cell cycle distribution in test groups of Celecoxib existed a significant difference（ P 〈0.05） and cell cycle distribution were significantly different among concentration groups （ P 〈 0.05 ）. Conclusion： The survival of Raji cells were inhibited by Celecoxib in vitro with a concentration and time dependent manners. Celecoxib induced cell cycle arrest in G1 phase and inhibited the growth of Raji cells, which may be one of the mechanisms of COX-2 independent pathway.
出处
《河南医学研究》
CAS
2010年第2期162-164,167,共4页
Henan Medical Research
